Immobilization of chiral enzyme inhibitors on solid supports by amide-forming coupling and olefin metathesis
Immobilization of chiral enzyme inhibitors on solid supports by amide-forming coupling and olefin metathesis
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DOI：
10.1016/s0040-4020(02)01052-9
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发表时间：
2002-10-14
期刊：
影响因子：
2.1
通讯作者：
Quax, WJ
中科院分区：
文献类型：
--
作者：
Reetz, MT;Rüggeberg, CJ;Quax, WJ
The question whether phage display can be used as a selection method in the directed evolution of enantioselective enzymes has not been answered satisfactorily to date. In order to be able to test this in a specific case, namely in the hydrolytic kinetic resolution of the acetate derived from alpha,beta- isopropylideneglycerol (IPG) catalyzed by the lipase from Bacillus subtilis, suicide enzyme inhibitors anchored on porous glass or polymer beads were designed and synthesized. These are immobilized phosphonates, which bear a leaving group and also contain the chiral substrate (D) and (L)-IPG, Modified SIRAN((R)) (porous glass) and Tentagel((R)) (polymer) were chosen as carriers, attachment occurring via amide-forming coupling or Ru-catalyzed olefin metathesis. Initial lipase inhibition studies are also reported. (C) 2002 Elsevier Science Ltd. All rights reserved.
