miR-373 suppresses gastric cancer metastasis by downregulating vimentin
miR-373 suppresses gastric cancer metastasis by downregulating vimentin
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miR-373通过下调波形蛋白抑制胃癌转移
DOI：
10.3892/mmr.2017.8291
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发表时间：
2018-03-01
影响因子：
3.4
通讯作者：
Xu, Wenrong
中科院分区：
文献类型：
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作者：
Shi, Yinghong;Shi, Hui;Xu, Wenrong
据报道，microRNA-373(miR-373)是一种癌基因，存在于许多人类实体肿瘤中。然而，miR-373在胃癌中的作用尚未完全阐明，其机制尚不清楚。在本研究中，我们通过逆转录-定量聚合酶链式反应比较了miR-373在临床胃癌组织和配对的非肿瘤组织中的表达。此外，还研究了miR-373对胃癌细胞增殖、迁移和侵袭的影响。用HSA-miR-373模拟物模拟内源性miR-373的功能。采用集落形成实验和流式细胞仪检测细胞增殖情况。采用创面愈合和Transwell侵袭实验检测胃癌细胞的迁移和侵袭能力。Western blotting检测上皮-间充质转化相关蛋白的表达。结果表明，miR-373在胃癌组织中的表达水平高于配对的非肿瘤组织。已证实miR-373通过下调Vimentin的表达抑制胃癌细胞系SGC-7901和HGC-27的迁移和侵袭。本研究结果表明miR-373在人胃癌的转移中起致癌作用，可能为胃癌的治疗提供新的策略。
MicroRNA-373 (miR-373) has been reported to be an oncogene in a number of solid human tumors. However, the role of miR-373 in gastric cancer has not been completely elucidated and the mechanisms remain unclear. In the present study, we compared miR-373 expression between clinical gastric cancer tissues and paired non-tumorous tissues by reverse transcription-quantitative polymerase chain reaction. The impact of miR-373 on proliferation, migration and invasion in gastric cancer cells was additionally investigated. Hsa-miR-373 mimics were applied to mimic the function of endogenous miR-373. A colony formation assay and flow cytometry were performed to analyze the proliferation of gastric cancer cells. Wound healing and Transwell invasion assays were employed to detect the migratory and invasive abilities of gastric cancer cells. Western blotting was used to test the expression of epithelial-mesenchymal transition-associated proteins. The results demonstrated that the level of miR-373 in gastric cancer was upregulated compared with paired non-tumorous tissues. It was confirmed that miR-373 inhibited the migration and invasion of the gastric cancer cell lines SGC-7901 and HGC-27 by downregulating vimentin expression. The results of the present study demonstrated an oncogenic role of miR-373 in the metastasis of human gastric cancer, and may provide a novel therapeutic strategy for gastric cancer.