Molecular cloning and sequence analysis of cDNA containing the entire coding region for human fetal liver cytochrome P-450.
Molecular cloning and sequence analysis of cDNA containing the entire coding region for human fetal liver cytochrome P-450.
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含有人胎肝细胞色素 P-450 整个编码区的 cDNA 的分子克隆和序列分析。
DOI：
10.1093/oxfordjournals.jbchem.a122632
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发表时间：
1989
影响因子：
2.7
通讯作者：
T. Kamataki
中科院分区：
文献类型：
--
作者：
M. Komori;K. Nishio;H. Ohi;M. Kitada;T. Kamataki
从人胎肝cDNA文库中，获得了含有与P-450 HFLa相关的细胞色素P-450形式的整个编码区的cDNA克隆（λ HFL 33）。该克隆长1，971 bp，具有1，509个核苷酸的开放阅读框，编码503个氨基酸的多肽。λ HFL 33的核苷酸和推导的氨基酸序列与NF 25和HLp cDNA非常相似，但明显不同于NF 25和HLp cDNA，NF 25和HLp cDNA编码成人肝脏中的细胞色素P-450形式。HFL 33蛋白的N-末端氨基酸序列与P-450 HFLa蛋白的N-末端氨基酸序列一致。
From a human fetal liver cDNA library, a cDNA clone (lambda HFL33) containing the entire coding region for a form of cytochrome P-450 related to P-450 HFLa was obtained. The clone was 1,971 bp long and had an open reading frame of 1,509 nucleotides coding for a 503 amino acid polypeptide. The nucleotide and the deduced amino acid sequences of lambda HFL33 were very similar to but clearly distinct from those of NF25 and HLp cDNAs, which code for forms of cytochrome P-450 in adult human liver. The deduced N-terminal amino acid sequence of the HFL33 protein was identical to that of P-450 HFLa.