RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts using a CoulArray detector
RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts using a CoulArray detector
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DOI：
10.1002/jssc.200500003
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发表时间：
2005-06-01
影响因子：
3.1
通讯作者：
Horna, A
中科院分区：
文献类型：
--
作者：
Jandera, P;Skeríková, V;Horna, A
建立了用梯度高效液相色谱-多通道电化学库仑检测法分析饮料和植物提取物中酚类和黄酮类天然抗氧化剂的方法。为此目的的各种反相柱的适用性进行了比较; pH和移动的相梯度进行了优化的分离选择性和检测灵敏度。由于各种样品类型中的目标化合物不同，因此使用先前描述的重叠分辨率图和归一化分辨率乘积方法来选择最佳柱和梯度，以适合各个样品类型的分析。将该方法应用于啤酒、葡萄酒、茶叶和雪莲果提取物中酚类化合物和黄酮类化合物的分析。共鉴定出32种酚类化合物，包括苯甲酸和肉桂酸的衍生物、黄酮类化合物及少量糖苷类化合物。八通道库仑阵列检测提供了高选择性和灵敏度，检测限在低μ g L-1范围内，至少比使用Coulochem检测器的单通道库仑检测低一个数量级。不需要特殊的样品预处理，并且由于CoulArray检测器与梯度洗脱的兼容性，不同极性的酚类抗氧化剂可以在一次运行中测定。除了保留时间之外，前主峰和后主峰的面积与主峰面积的比率可用于改进天然抗氧化剂的鉴定。
Methods were developed for the analysis of natural antioxidants including phenolic compounds and flavonoids in beverages and plant extracts using gradient HPLC with multi-channel electrochemical coulometric detection. Suitability of various reversed-phase columns for this purpose was compared; pH and mobile phase gradients were optimized with respect to the separation selectivity and sensitivity of detection. Because of different target compounds in various sample types, the overlapping resolution maps and the normalized resolution product approaches described earlier were used to select optimum columns and gradients to suit the analysis of the individual sample types. The methods were applied to the analysis of phenolic compounds and flavonoids in beer, wine, tea, and yacon extracts. 32 phenolic compounds were identified and determined, including derivatives of benzoic and cinnamic acids, flavones, and a few related glycosides. Eight-channel CoulArray detection offers high selectivity and sensitivity with limits of detection in the low mu g L-1 range, at least an order of magnitude lower than single-channel coulometric detection using the Coulochem detector. No special sample pretreatment is necessary and, because of the compatibility of the CoulArray detector with gradient elution, phenolic antioxidants of different polarities can be determined in a single run. In addition to the retention times, the ratios of the areas of the pre-dominant and post-dominant peaks to the area of the dominant peak can be used for improved identification of natural antioxidants.