The pattern of expression of protease-activated receptors (PARs) during early trophoblast development
The pattern of expression of protease-activated receptors (PARs) during early trophoblast development
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DOI：
10.1002/path.1338
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发表时间：
2003-05-01
影响因子：
7.3
通讯作者：
Bar-Shavit, R
中科院分区：
文献类型：
--
作者：
Even-Ram, SC;Grisaru-Granovsky, S;Bar-Shavit, R
人类胎儿的发育取决于胚胎进入母体循环的能力。因此，新形成的胎盘、滋养层的特化干细胞侵入子宫及其动脉网络，建立有效的胎儿-母体分子交换。为了完成这一任务，妊娠前三个月的滋养层分化涉及细胞增殖、侵袭和细胞外基质 (ECM) 重塑。滋养层侵袭与肿瘤细胞侵袭有许多共同特征，不同之处在于它在空间和时间上受到严格控制。我们之前已经证明，PAR1是蛋白酶激活受体（PAR）家族的第一个成员，在肿瘤细胞侵袭中发挥着核心作用。在本研究中，我们检查了 PAR1 和其他 PAR 家族候选者在早期人类胎盘发育过程中的表达模式。我们发现 PARI 和 PAR3 在妊娠第 7 周和第 10 周期间在空间上高度表达，但在第 12 周及之后则不然。同样，与正常年龄匹配胎盘中的最低水平相比，在完全性葡萄胎的细胞滋养层细胞中观察到 PARI 和 PAR3 的高表达水平。总之，我们的数据表明 PARI 和 PAR3 参与限制性和非限制性病理性滋养细胞侵袭。版权所有 (C) 2003 John Wiley Sons, Ltd.
Human fetal development depends on the ability of the embryo to gain access to the maternal circulation. Thus, specialized stem cells of the newly formed placenta, trophoblast, invade the uterus and its arterial network to establish an efficient feto-maternal molecular exchange. To accomplish this task, trophoblast differentiation during the first trimester of pregnancy involves cell proliferation, invasion, and extracellular matrix (ECM) remodelling. Trophoblast invasion shares many features with tumour cell invasion, with the distinction that it is strictly spatially and temporally controlled. We have previously demonstrated that PAR1, the first member of the protease-activated receptor (PAR) family, plays a central role in tumour cell invasion. In the present study we have examined the pattern of expression of PAR1 and other PAR family candidates during early human placental development. We show that PARI and PAR3 are highly and spatially expressed between the 7th and 10th weeks of gestation but not at the 12th week and thereafter. Likewise, high expression levels of PARI and PAR3 were observed in the cytotrophoblast cells of complete hydatidiform mole as compared to minimal levels in normal age-matched placenta. Together, our data suggest the involvement of PARI and PAR3 in restricted and unrestricted pathological trophoblast invasion. Copyright (C) 2003 John Wiley Sons, Ltd.