Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cell Culture Prevents UVB-Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts
Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cell Culture Prevents UVB-Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts
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人脂肪间充质干细胞培养物的条件培养基可预防 UVB 诱导的人角质形成细胞和真皮成纤维细胞的皮肤老化
DOI：
10.3390/ijms21010049
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发表时间：
2020-01-01
影响因子：
5.6
通讯作者：
Yi, Tae-Hoo
中科院分区：
文献类型：
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作者：
Li, Lu;Ngo, Hien T. T.;Yi, Tae-Hoo
人脂肪间充质干细胞条件培养液(ADSC-CM)含有多种细胞因子和生长因子，可促进多种组织器官的再生和修复。本研究观察了ADSC-CM对紫外线照射的人角质形成细胞系HaCaT和正常人真皮成纤维细胞(NHDF)的保护作用。研究发现，ADSC-CM可以调节早期UVB反应信号通路中信号分子的表达，包括丝裂原活化蛋白激酶(MAPKs)、激活蛋白1(AP-1)和核因子-kappaB(NF-kappa B)。此外，ADSC-CM可上调抗氧化反应元件(ARE)，如II期基因血红素加氧酶-1(HO-1)，增加胶原合成增强子基因转化生长因子-β(TGF-β)的表达。经ADSC-CM处理后，基质金属蛋白酶-1(MMP-1)和I型前胶原合成抑制物(IL-6)的表达也受到抑制。综上所述，我们的研究说明了ADSC-CM在基于细胞的模型中的抗光老化活性。
Human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) contains cytokines and growth factors that can facilitate the regeneration and repair of various tissues and organs. In the present study, the protective activity of ADSC-CM treatment was investigated in UVB-irradiated human keratinocyte cell line HaCaTs and normal human dermal fibroblasts (NHDFs). It was found that ADSC-CM can modulate the expression of the signaling molecules in the early UVB responsive signaling pathways, including mitogen activated protein kinases (MAPKs), activator protein 1 (AP-1), and nuclear factor kappa B (NF-kappa B). In addition, ADSC-CM treatment could upregulate antioxidant response element (ARE) such as phase II gene heme oxygenase-1 (HO-1) and increase the expression of collagen synthesis enhancer gene transforming growth factor-beta (TGF-beta). The expression of matrix metalloproteinase-1 (MMP-1) and procollagen type I synthesis inhibitors such as interleukin-6 (IL-6) was also found to be suppressed upon ADSC-CM treatment. Taken together, our study illustrates the anti-photoaging activities of ADSC-CM in cell-based models.