A novel deep-sea bacteriophage possesses features of Wbeta-like viruses and prophages
A novel deep-sea bacteriophage possesses features of Wbeta-like viruses and prophages
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一种新型深海噬菌体，具有Wbeta样病毒和原噬菌体的特征
DOI：
10.1007/s00705-020-04579-6
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发表时间：
2020-05-01
影响因子：
2.7
通讯作者：
Zeng, Runying
中科院分区：
文献类型：
--
作者：
Chen, Yulei;Guo, Xun;Zeng, Runying
病毒作为最丰富的生物实体，是海洋生态系统的主要参与者。然而，我们对深海中病毒-宿主相互作用和病毒生态学的了解仍然非常有限。本研究从深海沉积物中分离到一种新的噬菌体（命名为噬菌体BVE 2），该噬菌体感染蜡样芽孢杆菌（Bacillus cereus group）。噬菌体BVE 2在感染后1.5 h内引起宿主裂解。然而，在BVE 2基因组中存在两个整合酶编码基因，表明BVE 2也可能遵循温和的策略。噬菌体BVE 2的基因组长度约为20 kb，预计编码28种蛋白质。基因组和系统发育分析表明，BVE 2是一个高度嵌合的噬菌体，它继承了W β样病毒B的遗传特征。cereusprophages和它的主机，表明频繁的水平基因转移事件发生在其进化过程中。这项研究将有助于揭示Wbeta样病毒的进化历史，并提高我们对深海病毒多样性和病毒-宿主相互作用的理解。
As the most abundant biological entities, viruses are major players in marine ecosystems. However, our knowledge about virus-host interactions and viral ecology in the deep sea remains very limited. In this study, a novel bacteriophage (designated as phage BVE2) infectingBacillus cereusgroup bacteria, was isolated from deep-sea sediments. Phage BVE2 caused host lysis within 1.5 h after infection. However, the presence of two integrase-encoding genes in the BVE2 genome suggested that BVE2 may also follow a temperate strategy. The genome of phage BVE2 is approximately 20 kb in length and is predicted to encode 28 proteins. Genomic and phylogenetic analysis suggested that BVE2 is a highly mosaic phage that has inherited genetic features from Wbeta-like viruses,B. cereusprophages, and its host, suggesting that frequent horizontal gene transfer events occurred during its evolution. This study will help to reveal the evolutionary history of Wbeta-like viruses and improve our understanding of viral diversity and virus–host interactions in the deep sea.