Lam6 Regulates the Extent of Contacts between Organelles.
Lam6 Regulates the Extent of Contacts between Organelles.
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DOI：
10.1016/j.celrep.2015.06.022
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发表时间：
2015-07-07
期刊：
影响因子：
8.8
通讯作者：
Schuldiner M
中科院分区：
文献类型：
--
作者：
Elbaz-Alon Y;Eisenberg-Bord M;Shinder V;Stiller SB;Shimoni E;Wiedemann N;Geiger T;Schuldiner M
细胞器之间的通信对于真核细胞作为一个连贯的单位发挥作用至关重要。一种重要的通讯方式是通过膜接触位点，在那里两个细胞器非常接近，允许脂质和小溶质在它们之间运输。接触部位的大小是动态的，可以随着环境或细胞刺激而变化;然而，这是如何调节的还不清楚。在这里，我们表明，酿酒酵母Lam 6驻留在几个中央接触点：ERMES（ER/线粒体遇到结构），vCLAMP（空泡和线粒体补丁），和NVJ（核空泡连接）。我们表明，Lam 6是足够的生理条件下的接触部位的扩展和必要的协调接触部位的大小。鉴于Lam 6是一个大的蛋白质家族的一部分，并且在脊椎动物中是保守的，我们的工作为研究细胞器通信的基本原理开辟了途径。Lam 6定位于三个主要的细胞接触点：ERMES、vCLAMP和NVJ Lam 6是从酵母到人类保守的一种β-结构域蛋白Lam 6的过表达导致所有三个接触位点的扩增Lam 6对于ERMES和vCLAMP之间的串扰是必不可少的。发现酵母Lam 6，这是一个保守的蛋白质家族的一部分，被发现在三种类型的细胞器接触位点，ERMES，vCLAMP，和NVJ。Lam 6的过表达足以扩展这些接触位点，并且其表达对于接触位点大小的交叉调节是必需的。
Communication between organelles is crucial for eukaryotic cells to function as one coherent unit. An important means of communication is through membrane contact sites, where two organelles come into close proximity allowing the transport of lipids and small solutes between them. Contact sites are dynamic in size and can change in response to environmental or cellular stimuli; however, how this is regulated has been unclear. Here, we show that Saccharomyces cerevisiae Lam6 resides in several central contact sites: ERMES (ER/mitochondria encounter structure), vCLAMP (vacuole and mitochondria patch), and NVJ (nuclear vacuolar junction). We show that Lam6 is sufficient for expansion of contact sites under physiological conditions and necessary for coordination of contact site size. Given that Lam6 is part of a large protein family and is conserved in vertebrates, our work opens avenues for investigating the underlying principles of organelle communication. Lam6 is localized to three major cellular contacts: ERMES, vCLAMP, and NVJ Lam6 is a GRAM domain protein conserved from yeast to humans Overexpression of Lam6 results in the expansion of all three contact sites Lam6 is essential for the cross-talk between ERMES and vCLAMP Elbaz-Alon et al. find that yeast Lam6, which is part of a conserved protein family, is found in three types of organellar contact sites, ERMES, vCLAMP, and NVJ. Overexpression of Lam6 is sufficient to expand these contact sites and its expression is necessary for cross-regulation of contact site size.