Cloning of rat and mouse P2Y purinoceptors.
Cloning of rat and mouse P2Y purinoceptors.
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大鼠和小鼠 P2Y 嘌呤受体的克隆。
DOI：
10.1006/bbrc.1995.1798
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Bell,GI
中科院分区：
文献类型：
--
作者：
Tokuyama,Y;Hara,M;Jones,EM;Fan,Z;Bell,GI
细胞对 ATP 的反应由称为 P2 嘌呤受体的特定高亲和力受体介导，其中的五个亚类已在药理学上定义为 - P2X、P2Y、P2U、P2T 和 P2Z。从胰岛素瘤 cDNA 文库中分离出编码大鼠 P2Y 嘌呤受体的 cDNA 克隆。 373个氨基酸的大鼠P2Y嘌呤受体序列与鸡P2Y和大鼠P2U嘌呤受体分别具有85.7%和37.8%的同一性。还测定了小鼠P2Y嘌呤受体的序列，与相应的大鼠序列有97.1%的氨基酸同一性。 RNA印迹研究表明，大鼠P2Y嘌呤受体mRNA在许多组织中以不同水平表达，包括心脏、脑、脾、肺、肝、骨骼肌和肾，但在睾丸中未检测到。克隆的大鼠 P2Y 嘌呤受体在非洲爪蟾卵母细胞中表达，并具有该受体亚型的预期特性。
The cellular responses to ATP are mediated by specific high-affinity receptors designated as P2purinoceptors, five subclasses of which have been defined pharmacologically - P2X, P2Y, P2U, P2T, and P2Z. A cDNA clone encoding a rat P2Ypurinoceptor was isolated from an insulinoma cDNA library. The 373-amino acid rat P2Ypurinoceptor sequence has 85.7% and 37.8% identity with chicken P2Yand rat P2Upurinoceptors, respectively. The sequence of the mouse P2Ypurinoceptor was also determined and there was 97.1% amino acid identity with the corresponding rat sequence. RNA blotting studies showed that rat P2Ypurinoceptor mRNA was expressed at variable levels in many tissues including heart, brain, spleen, lung, liver, skeletal muscle and kidney, although it was not detected in testis. The cloned rat P2Ypurinoceptor was expressed in Xenopus laevis oocytes and possessed the properties expected for this receptor subtype.