Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome
Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome
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DOI：
10.1128/jcm.44.3.888-891.2006
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发表时间：
2006-03-01
影响因子：
9.4
通讯作者：
Palzkill, T
中科院分区：
文献类型：
--
作者：
Brinkman, MB;McKevitt, M;Palzkill, T
为了鉴定在人类对梅毒的免疫应答中重要的抗原，用梅毒螺旋体梅毒亚种蛋白质组中的1，039种蛋白质中的908种检查了梅毒患者的血清抗体反应性。苍白球使用蛋白质阵列酶联免疫吸附测定。34个蛋白质表现出显着的反应性时，与人血清中的梅毒早期潜伏期的患者进行测定。对鉴定的抗原亚组进一步检查了在原发、继发和潜伏期疾病阶段的抗体反应性，结果表明对个体T.苍白球蛋白在感染的时间过程中以不同的速率发展。
To identify antigens important in the human immune response to syphilis, the serum antibody reactivity of syphilitic patients was examined with 908 of the 1,039 proteins in the proteome of Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay. Thirty-four proteins exhibited significant reactivity when assayed with human sera from patients in the early latent stage of syphilis. A subset of antigens identified were further scrutinized for antibody reactivity at primary, secondary, and latent disease stages, and the results demonstrate that the humoral immune response to individual T. pallidum proteins develops at different rates during the time course of infection.