Hydrogen supplementation of preservation solution improves viability of osteochondral grafts.
Hydrogen supplementation of preservation solution improves viability of osteochondral grafts.
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DOI：
10.1155/2014/109876
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发表时间：
2014
影响因子：
--
通讯作者：
Takaso M
中科院分区：
文献类型：
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作者：
Yamada T;Uchida K;Onuma K;Kuzuno J;Ujihira M;Inoue G;Sato B;Kurokawa R;Sakai R;Takaso M
同种异体骨软骨组织（OCT）用于治疗大型软骨缺损。通常，在疾病筛选期间收集的OCT保存在4°C下;然而，冷保存期间细胞活力的逐渐降低对移植结果产生不利影响。因此，需要改进维持OCT细胞活力的储存方法，以增加高质量OCT的可用性并改善治疗结果。在这里，我们评估了长期氢输送到保存溶液中是否改善了大鼠OCT在冷保存过程中的生存力。与未补充培养基相比，补充氢的Dulbecco改良Eagles培养基（DMEM）和威斯康星州大学（UW）溶液均显著提高了OCT在4°C保存21天期间的细胞活力。然而，OCT在含氢DMEM中的长期冷保存与软骨细胞的活力和形态方面的最佳维持相关。我们的研究结果表明，保存在补充有氢的DMEM中的OCT是一种有前途的材料，用于修复临床环境中的大型软骨缺损。
Allogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefore, improved storage methods that maintain the cell viability of OCTs are needed to increase the availability of high-quality OCTs and improve treatment outcomes. Here, we evaluated whether long-term hydrogen delivery to preservation solution improved the viability of rat OCTs during cold preservation. Hydrogen-supplemented Dulbecco's Modified Eagles Medium (DMEM) and University of Wisconsin (UW) solution both significantly improved the cell viability of OCTs during preservation at 4°C for 21 days compared to nonsupplemented media. However, the long-term cold preservation of OCTs in DMEM containing hydrogen was associated with the most optimal maintenance of chondrocytes with respect to viability and morphology. Our findings demonstrate that OCTs preserved in DMEM supplemented with hydrogen are a promising material for the repair of large cartilage defects in the clinical setting.