Human SWI-SNF component BRG1 represses transcription of the c-fos gene.
Human SWI-SNF component BRG1 represses transcription of the c-fos gene.
复制标题
人类 SWI-SNF 成分 BRG1 抑制 c-fos 基因的转录。
DOI：
10.1128/mcb.19.4.2724
复制
发表时间：
1999
影响因子：
5.3
通讯作者：
Engel,DA
中科院分区：
文献类型：
--
作者：
Murphy,DJ;Hardy,S;Engel,DA
Yeast and mammalian SWI-SNF complexes regulate transcription through active modification of chromatin structure. Human SW-13 adenocarcinoma cells lack BRG1 protein, a component of SWI-SNF that has a DNA-dependent ATPase activity essential for SWI-SNF function. Expression of BRG1 in SW-13 cells potentiated transcriptional activation by the glucocorticoid receptor, which is known to require SWI-SNF function. BRG1 also specifically repressed transcription from a transfected c-fospromoter and correspondingly blocked transcriptional activation of the endogenous c-fosgene. Mutation of lysine residue 798 in the DNA-dependent ATPase domain of BRG1 significantly reduced its ability to repress c-fostranscription. Repression by BRG1 required the cyclic AMP response element of the c-fospromoter but not nearby binding sites for Sp1, YY1, or TFII-I. Using human C33A cervical carcinoma cells, which lack BRG1 and also express a nonfunctional Rb protein, transcriptional repression by BRG1 was weak unless wild-type Rb was also supplied. Interestingly, Rb-dependent repression by BRG1 was found to take place through a pathway that is independent of transcription factor E2F.
