Characterization of an anti-FLAG antibody binding protein in V. cholerae.
Characterization of an anti-FLAG antibody binding protein in V. cholerae.
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霍乱弧菌中抗 FLAG 抗体结合蛋白的表征。
DOI：
10.1016/j.bbrc.2020.05.169
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发表时间：
2020
影响因子：
3.1
通讯作者：
Dörr,Tobias
中科院分区：
文献类型：
--
作者：
Shin,Jung-Ho;Lanz,Michael;Smolka,MarcusB;Dörr,Tobias
flag标签通常用于蛋白质丰度测量和活细胞中蛋白质-蛋白质相互作用的鉴定。我们观察到霍乱病原体霍乱弧菌编码一种flag抗体反应蛋白，并鉴定该蛋白为外膜孔蛋白Porin4，其序列与3xFLAG表位标签非常相似。我们已经证明了Porin4中的保守肽序列（称为Porin4标签）与单克隆抗flag M2抗体的结合亲和力。此外，我们还构建了aporin4缺失突变体，该突变体可用于无背景FLAG抗体检测实验。
FLAG-tags are commonly used for protein abundance measurements and for identification of protein-protein interactions in living cells. We have observed that the cholera pathogenVibrio choleraeencodes a FLAG-antibody-reactive protein and identified this protein as an outer membrane porin, Porin4, which contains a sequence very similar to the 3xFLAG epitope tag. We have demonstrated the binding affinity of the conserved peptide sequence (called Porin 4 tag) in Porin4 against monoclonal anti-FLAG M2 antibody. In addition, we created aporin4deletion mutant, which can be used for background-less FLAG antibody detection experiments.