ESTIMATION OF GLOMERULAR VOLUME - A COMPARISON OF 4 METHODS
ESTIMATION OF GLOMERULAR VOLUME - A COMPARISON OF 4 METHODS
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DOI：
10.1038/ki.1992.165
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发表时间：
1992-04-01
影响因子：
19.6
通讯作者：
MAUER, SM
中科院分区：
文献类型：
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作者：
LANE, PH;STEFFES, MW;MAUER, SM
对来自 10 名胰岛素依赖型糖尿病患者和 6 名正常肾脏捐献者的 Zenkerfixed 石蜡包埋活组织检查中估计肾小球体积的方法进行了比较。 使用两种测量个体肾小球体积的方法：卡瓦列里法（被认为是“金标准”）和最大轮廓面积（MPA）法。 还研究了Weibel和Gomez的方法以及基于disector原理的方法；两者都估计平均体积 (V(G))。 MPA 和 Cavalieri 显示出很强的相关性（r = 0.93；P < 0.001），尽管 MPA 方法始终高估了真实体积；可靠估计 V(G) 需要六个肾小球。 Disector 方法与 Cavalieri 确定的 V(G) 不相关。 Weibel-Gomez 确实与 Cavalieri 相关（r = 0.68；P < 0.05），但高估了 V(G)。 Weibel-Gomez 至少需要 15 个剖面才能提供 V(G) 的可靠估计。 Cavalieri、MPA 和 Weibel-Gomez 方法都可以提供 V(G) 的可靠估计，后两种方法具有适当的校正因子。 个体肾小球体积方法虽然更耗时，但提供了有关肾小球群体变化和分布的信息，是肾小球体积研究的首选方法。
Methods for estimating glomerular volume were compared in Zenkerfixed, paraffin-embedded biopsies from 10 patients with insulin-dependent diabetes mellitus and 6 normal kidney donors. Two methods of measurement of individual glomerular volumes were used: the Cavalieri method (considered the "gold standard") and the maximal profile area (MPA) method. Also studied were the method of Weibel and Gomez and a method based on the disector principle; both estimate mean volume (V(G)). MPA and Cavalieri showed strong correlation (r = 0.93; P < 0.001), although the MPA method consistently overestimated the true volume; six glomeruli were necessary for a reliable estimate of V(G). The disector method did not correlate with V(G) determined by Cavalieri. Weibel-Gomez did correlate with Cavalieri (r = 0.68; P < 0.05), but overestimated V(G). At least 15 profiles were needed to provide a dependable estimate of V(G) by Weibel-Gomez. The Cavalieri, MPA, and Weibel-Gomez methods all can provide reliable estimates of V(G), the latter two with appropriate correction factors. The individual glomerular volume methods, while more time consuming, provide information on variation and distribution of the glomerular population and are the methods of choice for studies of glomerular volume.