Double bouquet cell axons in the human temporal neocortex: relationship to bundles of myelinated axons and colocalization of calretinin and calbindin D-28k immunoreactivities
Double bouquet cell axons in the human temporal neocortex: relationship to bundles of myelinated axons and colocalization of calretinin and calbindin D-28k immunoreactivities
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DOI：
10.1016/s0891-0618(97)00050-1
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发表时间：
1997-10-01
影响因子：
2.8
通讯作者：
DeFelipe, J
中科院分区：
文献类型：
--
作者：
delRio, MR;DeFelipe, J
我们已经研究了双花束细胞轴突的分布，免疫细胞化学染色的钙结合蛋白calretinin和calbindin D-28 k在人类颞叶新皮质，在有关束的有髓轴突（来自锥体细胞）和这些钙结合蛋白的共定位。在皮层III-V层的切向切片中，双束细胞轴突的大量分布和规律性清晰可见。在这些切片中，我们估计每10，000 μ m（2）为11.65 ± 0.44，平均直径为12.10 ± 0.63 μ m，平均中心间距为29.8 ± 0.91 μ m。这些值与先前在猴新皮层中报告的值非常相似。双束细胞轴突的分布与有髓轴突束的分布密切相关，两者有重叠，基本上一一对应。最后，双标记免疫荧光实验表明，绝大多数的双束细胞轴突免疫反应的钙结合蛋白也染色钙视网膜蛋白。由于相对较少的细胞胞体在人类颞叶皮层双标记，我们的结论是，双花束细胞可能代表了一个显着的亚群的神经元共定位这些钙结合蛋白。(C)1997年Elsevier Science B.V.
We have examined the distribution of double bouquet cell axons, immunocytochemically stained for the calcium-binding proteins calretinin and calbindin D-28k in the human temporal neocortex, in relation to bundles of myelinated axons (originating from pyramidal cells) and the colocalization of these calcium-binding proteins. The large number and regularity of distribution of double bouquet cell axons was clearly visualized in tangential sections from cortical layers III-V. In these sections, we estimated that the mean number +/- standard deviation of double bouquet cell axons per 10,000 mu m(2) was 11.65 +/- 0.44 with a mean diameter of 12.10 +/- 0.63 mu m and a mean center-to-center spacing of 29.8 +/- 0.91 mu m. These values are very similar to those previously reported in the monkey neocortex. The distribution of double bouquet cell axons was closely related to bundles of myelinated axons; there was overlapping with basically a one-to-one correspondence. Finally, double-label immunofluorescence experiments revealed that the vast majority of double bouquet cell axons immunoreactive for calbindin were also stained for calretinin. Since relatively few cell somata were double-labeled in the human temporal cortex, we concluded that double bouquet cells may represent a significant subpopulation of neurons that colocalize these calcium-binding proteins. (C) 1997 Elsevier Science B.V.