Identification of bacterial strains by laboratories participating in the Deutsches Krebsforschungszentrum programme quality assurance
Identification of bacterial strains by laboratories participating in the Deutsches Krebsforschungszentrum programme quality assurance
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DOI：
10.1258/002367707782314256
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发表时间：
2007-10-01
影响因子：
2.4
通讯作者：
Reubsaet, F. A. G.
中科院分区：
文献类型：
--
作者：
Boot, R.;Reubsaet, F. A. G.
德国实验动物中心（DKFZ）的质量保证计划（QAP）是为服务实验动物学科而开发的能力测试系统。QAP包括来自不同动物物种的两种细菌菌株的季度分布，用于物种水平的鉴定和抗生素敏感性测试。我们将1996-2004年QAP参与者报告的鉴定结果与荷兰细菌诊断参考实验室获得的68个样品的鉴定结果进行了比较，这些样品包括71种细菌菌株和一种真菌。当根据革兰氏染色的形态学和来源（动物与环境，啮齿动物和兔子与其他动物物种，病原体与非病原体）将细菌分配到不同的组时，报告和正确鉴定的频率存在显著差异。啮齿动物和家兔病原体的正确率为73%，而所有菌株的正确率仅为60%。我们假设大多数QAP参与者来自实验动物诊断实验室。如果这是真的，实验动物学科的实验室正确识别细菌种类的能力远远低于人类诊断实验室认为可接受的限度。培养细菌的分布绕过了动物微生物监测中最困难的一步，即来自临床样本的原代培养。我们建议建立一个QAP，其中包括模拟通常提交给实验动物诊断实验室的临床样本的标本分布。
The quality assurance programme (QAP) of the Deutsches Krebsforschungszentrum (DKFZ) is a proficiency testing system developed to service the laboratory animal discipline. QAP comprises the quarterly distribution of two bacterial strains originating from various species of animals for identification to the species level and antibiotic susceptibility testing. We compared identification results reported by QAP participants over the years 1996-2004 with those obtained by the Dutch Bacterial Diagnostics reference laboratory on 68 samples comprising 71 bacterial strains and a fungus. Significant differences were found in the frequency of reported and correct identifications when bacteria were assigned to different groups based on morphology by Gram stain and on origin (animal versus environmental, rodent and rabbit versus other animal species, pathogen versus non-pathogens). Rodent and rabbit pathogens yielded 73% correct identifications, and with all bacterial strains only 60% of the identifications were correct. We assume that most QAP participants were from laboratory animal diagnostic laboratories. If this is true, the capabilities of laboratories in the laboratory animal discipline to correctly identify bacterial species are well below what are considered acceptable limits for human diagnostic laboratories. The distribution of cultured bacteria circumvents the most difficult step in the microbiological monitoring of animals, namely primary culture from clinical samples. We propose to set up a QAP that comprises the distribution of specimens mimicking clinical samples normally submitted to laboratory animal diagnostic laboratories.