Bile salt micellar electrokinetic chromatography of bilirubin and related compounds.
Bile salt micellar electrokinetic chromatography of bilirubin and related compounds.
复制标题
胆红素及相关化合物的胆盐胶束电动色谱。
DOI：
10.1016/s0021-9673(97)00426-3
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Bushey,MM
中科院分区：
文献类型：
--
作者：
Nittler,MP;Desai,RA;Salikof,DA;Kurtin,WE;Bushey,MM
通过胶束电动色谱 (MEKC) 研究了胆红素、胆绿素、胆红素二甲酯、胆绿素二甲酯、黄胆红酸和黄胆红素甲酯与三羟基和二羟基胆盐溶液的相互作用。每种化合物的容量因子是在 pH 范围 6.5-9.0 的不同胆汁盐溶液中测量的。在所有胆汁盐溶液中，当 pH 值低于 7 时，胆红素的容量因子都会增加。胆绿素和黄胆红素对所有胆汁盐表现出基本相同的容量因子。胆绿素二甲酯和黄胆红素甲酯在三羟基胆盐中也具有非常相似的容量因子，其大于其类似物。这些酯的容量因子在二羟基胆盐中较高，胆绿素二甲酯的容量因子是黄胆红素甲酯的两倍。讨论了这些化合物的 MEKC 分析中涉及的因素。
The interaction of bilirubin, biliverdin, bilirubin dimethyl ester, biliverdin dimethyl ester, xanthobilirubic acid, and xanthobilirubin methyl ester with trihydroxy and dihydroxy bile salt solutions is investigated by micellar electrokinetic chromatography (MEKC). The capacity factor of each compound is measured in solutions of the different bile salts over the pH range of 6.5–9.0. The capacity factor of bilirubin increases with pH below 7 in all bile salt solutions. Biliverdin and xanthobilirubin show essentially identical capacity factors for all bile salts. Biliverdin dimethyl ester and xanthobilirubin methyl ester also have very similar capacity factors, which are greater than those of the car☐y analogs, in trihydroxy bile salts. The capacity factors of these esters are higher in the dihydroxy bile salts, with the capacity factor of biliverdin dimethyl ester being twice that of xanthobilirubin methyl ester. Factors involved in the MEKC analysis of these compounds are discussed.