Allelic Variation in a Single Genomic Region Alters the Microbiome of the Snail Biomphalaria glabrata
Allelic Variation in a Single Genomic Region Alters the Microbiome of the Snail Biomphalaria glabrata
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DOI：
10.1093/jhered/esy014
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发表时间：
2018-07-01
影响因子：
3.1
通讯作者：
Blouin, Michael S.
中科院分区：
文献类型：
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作者：
Allan, Euan R. O.;Tennessen, Jacob A.;Blouin, Michael S.
淡水蜗牛是许多寄生虫的中间宿主，这些寄生虫可能对人类健康和农业产生负面影响。了解这些疾病的传播需要更完整的蜗牛宿主的免疫生物学特性。这包括其微生物组和遗传因素的表征，这些因素可能与这一重要的生物群落相互作用。在瓜德罗普抗性复合体（GRC）的基因组区域的瓜德罗普双脐螺光滑的等位基因变异影响他们的易感性，以介体感染，并可能有其他的作用，在蜗牛的免疫反应。在本研究中，我们研究了蜗牛的GRC基因型是否在塑造细菌多样性和组合物上或整个蜗牛中发挥作用。我们发现，GRC单倍型，包括耐药基因型，有一个显着的影响，存在于或对整个蜗牛的细菌物种的多样性，包括相对丰度的Gemmatimonas aurantiaca和Micavibrio aerosavorus。这些发现支持了这一假设，即GRC区域可能参与了可以改变这些蜗牛的微生物群落的途径，并且可能在B中具有更多的免疫作用。比原来认为的更光滑。这也是少数几个例子之一，其中特定基因座的等位基因变异已被证明会影响任何物种的微生物组。
Freshwater snails are the intermediate hosts for numerous parasitic worms which can have negative consequences for human health and agriculture. Understanding the transmission of these diseases requires a more complete characterization of the immunobiology of snail hosts. This includes the characterization of its microbiome and genetic factors which may interact with this important commensal community. Allelic variation in the Guadeloupe resistance complex (GRC) genomic region of Guadeloupean Biomphalaria glabrata influences their susceptibility to schistosome infection and may have other roles in the snail immune response. In the present study, we examined whether a snail's GRC genotype has a role in shaping the bacterial diversity and composition present on or in whole snails. We show that the GRC haplotype, including the resistant genotype, has a significant effect on the diversity of bacterial species present in or on whole snails, including the relative abundances of Gemmatimonas aurantiaca and Micavibrio aeruginosavorus. These findings support the hypothesis that the GRC region is likely involved in pathways that can modify the microbial community of these snails and may have more immune roles in B. glabrata than originally believed. This is also one of few examples in which allelic variation at a particular locus has been shown to affect the microbiome in any species.