HUMAN CORONAVIRUS OC43 INTERACTS WITH MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I MOLECULES AT THE CELL-SURFACE TO ESTABLISH INFECTION
HUMAN CORONAVIRUS OC43 INTERACTS WITH MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I MOLECULES AT THE CELL-SURFACE TO ESTABLISH INFECTION
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DOI：
10.3109/08820139409066826
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发表时间：
1994-01-01
影响因子：
2.8
通讯作者：
COLLINS, AR
中科院分区：
文献类型：
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作者：
COLLINS, AR
人类冠状病毒与普通感冒、腹泻和小肠结肠炎有关，并与多发性硬化症有关。HLA I类分子可能作为OC43受体发挥关键作用，因为针对HLA- a、-B和-C特异性的单克隆抗体（mAb）W6/32完全阻断了人横纹肌肉瘤（RD）细胞的感染性。用稳定转染HLA- a3.1的人浆细胞和未转染的HMY检测HLA 1类抗原作为病毒受体的作用。C1R细胞不表达HLA-A和-B分子。当细胞（5 × 10(6)）以1的倍数感染时，HLA。A3转染的细胞产生10(8)个PFU的病毒，而在HMY中没有发生复制。C1R cells mAb W6/32使HMY细胞膜的病毒产量降低了99.9%。C1R HMY。A3细胞和鸡红细胞作为活细胞进行生物素化处理。用多克隆抗病毒抗体免疫沉淀检测生物素化细胞膜与病毒的结合，结果显示生物素化的HMY。在SDS-PAGE凝胶电泳、电印迹和avidin -辣根过氧化物酶染色时，A3膜与病毒抗体复合物共沉淀。这些结果为OC43病毒能够识别HLA I类作为细胞表面受体提供了直接证据。
Human coronaviruses have been associated with common colds, diarrhea and enterocolitis, and have been implicated in multiple sclerosis. HLA class I molecules may play a critical role as receptor for OC43 because monoclonal antibody (mAb)W6/32 to HLA-A, -B and -C specificities completely blocks infectivity in human rhabdomyosarcoma (RD) cells. The role of HLA class 1 antigen as the virus receptor was examined using HLA-A3.1 stably transfected human plasma cells and untransfected HMY.C1R cells which do not express HLA-A and -B molecules. When the cells (5x10(6)) were infected at a multiplicity of one, the HLA.A3 transfected cells produced 10(8) PFU of virus whereas no replication occurred in the HMY.C1R cells mAb W6/32 reduced the virus yield by 99.9% Cell membranes from HMY.C1R, HMY.A3 cells and chicken erythrocytes were biotinylated as live cells. Immunoprecipitation with polyclonal antiviral antibody to detect binding of biotinylated cell membranes to virus revealed that biotinylated HMY.A3 membranes coprecipitated with virus-antibody complexes when the immunoprecipitates were electrophoresed on SDS-PAGE gel, electroblotted and stained with Avidin-horseradish peroxidase. The results provide direct evidence that OC43 virus can recognize HLA class I as receptor on the cell surface.