Chemical and physical differentiation of superoxide dismutases in anaerobes.
Chemical and physical differentiation of superoxide dismutases in anaerobes.
复制标题
厌氧菌中超氧化物歧化酶的化学和物理分化。
DOI：
10.1128/jb.144.3.967-974.1980
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发表时间：
1980
影响因子：
3.2
通讯作者：
Dapper,CH
中科院分区：
文献类型：
--
作者：
Gregory,EM;Dapper,CH
几种专性厌氧菌类杆菌的粗提物或部分纯化的细胞提取物中的超氧化物歧化酶活性可被NaN3瞬时抑制，并在与H_2O_2孵育后迅速失活。NaN3的抑制程度从41%到93%不等，在5 mM H_2O_2中酶活性的半衰期从1.2到6.1min，取决于生物体试验。脆弱类杆菌(VPI 2393)在含有59Fe的特定培养基中生长时，在类似的生长条件下将放射性标记掺入对NaN3和H_2O_2敏感的40,000分子量的超氧化物歧化酶中，但不将54Mn掺入该蛋白质中。纳氏厌氧放线菌(VPI 9985)将54MN而不是59Fe结合到NaN3不敏感和抗H_2O_2的超氧化物歧化酶中。该放线菌和其他几种放线菌超氧化物歧化酶的表观分子量。估计在11万到14万之间。将这些数据与均相金属超氧化物歧化酶的研究相比较，表明类杆菌属(Bacteroids spp.)所研究的含有一种亚铁过氧化物酶，而放线菌。含有一种亚麻黄超氧化物歧化酶。
Superoxide dismutase activity in crude or partially purified cell extracts from several species and strains of obligate anaerobe Bacteroides was inhibited instantaneously by NaN3 and was inactivated rapidly upon incubation with H2O2. The extent of NaN3 inhibition varied from 41 to 93%, and the half-life of the enzymatic activity in 5 mM H2O2 ranged from 1.2 to 6.1 min, depending upon the organism tests. When grown in a defined medium containing 59Fe, Bacteroides fragilis (VPI 2393) incorporated radiolabel into a 40,000-molecular-weight NaN3- and H2O2-sensitive superoxide dismutase but did not incorporate 54Mn into that protein under similar growth conditions. The anaerobe Actinomyces naeslundii (VPI 9985) incorporated 54Mn but not 59Fe into a NaN3-insensitive and H2O2-resistant superoxide dismutase. The apparent molecular weight of the superoxide dismutase from this and several other Actinomyces spp. was estimated to be 110,000 to 140,000. Comparison of these data with studies of homogeneous metallosuperoxide dismutases suggests that the Bacteroides spp. studied contain a ferrisuperoxide dismutase, whereas Actinomyces spp. contain a managanisuperoxide dismutase.