Optical measurements of invasive forces exerted by appressoria of a plant pathogenic fungus
Optical measurements of invasive forces exerted by appressoria of a plant pathogenic fungus
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DOI：
10.1126/science.285.5435.1896
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发表时间：
1999-09-17
期刊：
影响因子：
56.9
通讯作者：
Bastmeyer, M
中科院分区：
文献类型：
--
作者：
Bechinger, C;Giebel, KF;Bastmeyer, M
许多植物病原真菌，如谷物病原菌炭疽杆菌，分化出高度特化的感染结构，称为附着胞，它向底层的植物细胞发送穿透钉。附着胞已被证明能产生巨大的膨胀压力，但真菌对植物机械感染的直接证据尚缺乏。提出了一种利用弹性光波导对单个附着体局部施加的力进行可视化测量的显微方法。用该方法测定了稻瘟病菌寄主施加的力约为17微牛顿。
Many plant pathogenic fungi, such as the cereal pathogen Colletotrichum graminicola, differentiate highly specialized infection structures called appressoria, which send a penetration peg into the underlying plant cell. Appressoria have been shown to generate enormous turgor pressure, but direct evidence for mechanical infection of plants by fungi is Lacking. A microscopic method was developed that uses elastic optical waveguides to visualize and measure forces Locally exerted by single appressoria. By this method, the force exerted-by appressoria of C. graminicola was found to be about 17 micronewtons.