Study of bone repair mediated by recombination BMP-2/recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite
Study of bone repair mediated by recombination BMP-2/recombination CXC chemokine Ligand-13-loaded hollow hydroxyapatite microspheres/chitosan composite
复制标题
重组BMP-2/重组CXC趋化因子Ligand-13负载空心羟基磷灰石微球/壳聚糖复合材料介导的骨修复研究。
DOI：
10.1016/j.lfs.2019.116743
复制
发表时间：
2019-10-01
期刊：
影响因子：
6.1
通讯作者：
Liu，Shiwei
中科院分区：
文献类型：
--
作者：
Zeng，Jianhua;Xiong，Shilang;Liu，Shiwei
目的研究载重组骨形成蛋白2（rhBMP-2）/重组趋化因子配体13（rhCXCL 13）的空心羟基磷灰石（HA）微球/壳聚糖（CS）复合物的骨修复机制。第二，检测rhBMP-2缓释液对大鼠骨髓间充质干细胞ALP活性的影响和rhCXCL 13缓释液对大鼠骨髓间充质干细胞迁移的影响。第三，进行成骨细胞分化试验、X线评分和三点弯曲试验。结果：rhBMP-2能显著促进大鼠骨髓间充质干细胞的增殖和分化，RhCXCL 13能促进大鼠骨髓间充质干细胞的迁移。ALP活性和钙盐沉积检测表明rhBMP-2和rhCXCL 13能显著提高细胞的生物学活性和促进细胞分化能力。桡骨X线评分和抗弯强度试验表明，rhBMP-2和rhCXCL 13能促进骨愈合，提高骨组织的抗弯能力。RT-PCR和WB等体外分子生物学实验进一步证实了rhBMP-2和rhCXCL 13在骨形成和骨修复中的作用，提示空心HA微球/CS复合材料可作为rhBMP-2和rhCXCL 13的载体，有效地促进骨再生和骨修复。在此过程中，rhBMP-2可能通过调节rhCXCL 13募集的骨髓间充质干细胞促进骨再生。
AimsThe present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.Materials and methodsFirstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated. Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested. Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed. Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively.Key findingsRhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs. Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability. X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue. The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair.SignificanceOur results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair. In this process, rhBMP-2 may promote bone regeneration by regulating bone marrow MSCs cells recruited by rhCXCL13.