Quantitative analysis of adhesion-mediated cell migration in three-dimensional gels of RGD-grafted collagen
Quantitative analysis of adhesion-mediated cell migration in three-dimensional gels of RGD-grafted collagen
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DOI：
10.1114/1.259
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发表时间：
2000-01-01
影响因子：
3.8
通讯作者：
Dickinson, RB
中科院分区：
文献类型：
--
作者：
Burgess, BT;Myles, JL;Dickinson, RB
粘附介导的迁移在许多生理和病理过程中是必需的。进一步定量了解细胞迁移和细胞-基质粘附性之间的关系可能有助于治疗或组织工程应用。这项工作的目的是量化的三维细胞迁移作为一个功能的增加细胞基质的胶原蛋白凝胶内重建。通过将含有良好表征的甘氨酸-甘氨酸-天冬氨酸序列的额外粘附肽接枝到胶原蛋白来控制细胞基质粘附。以自动化的方式在延长的时间段内真实的时间跟踪多个单个细胞的膝关节维迁移。细胞位移进行统计分析，并拟合相关的持续随机游走模型，以估计均方根速度，定向持续时间，和随机运动系数。模型参数估计的基础上，细胞速度被发现是一个单调递减的函数，增加基层的粘附性，而定向的持续时间和随机运动系数表现出双相依赖性，最大值在大约中间浓度的接枝粘附肽，因此中间细胞-基层粘附性。总之，这些研究表明，一个最佳的三维随机迁移，与以前的研究在二维表面上一致。然而，在随机运动的最大值对应于最大的方向持久性，而不是在细胞的速度。(C)2000生物医学工程学会：[S 0090 -6963（00）05001-3]。
Adhesion-mediated migration is required in a number of physiological and pathological processes. A further quantitative understanding of the relationship between cell migration and cell-substratum adhesiveness may aid in therapeutic or tissue engineering applications. The aim of this work was to quantify three-dimensional cell migration as a function of increasing cell-substratum adhesiveness within reconstituted collagen gels. Cell-substratum adhesiveness was controlled by grafting additional adhesive peptides containing the well-characterized arginine-glycine-aspartic acid sequence to collagen. The rkne-dimensional migration of multiple individual cells was tracked in real time in an automated fashion for extended periods. Cell displacements were statistically analyzed and fit to a correlated persistent random walk model to estimate root-mean-square speed, directional persistence time, and random motility coefficient. Based on model parameter estimates, cell speed was found to be a monotonically decreasing function of increasing substratum adhesiveness, while the directional persistence time and random motility coefficient exhibited a biphasic dependence, with maximum values at approximately intermediate concentrations of grafted adhesive peptide and hence intermediate cell-substratum adhesiveness. In conclusion, these studies suggest an optimal adhesiveness for three-dimensional random migration, consistent with previous studies on two-dimensional surfaces. However, the maximum in random motility corresponded to a maximum in directional persistence, not in cell speed. (C) 2000 Biomedical Engineering Society: [S0090-6963(00)05001-3].