Prefix/Suffix Variation in Retinoic Acid Response Elements
Prefix/Suffix Variation in Retinoic Acid Response Elements
复制标题
视黄酸响应元件的前缀/后缀变化
DOI：
10.1145/3388440.3414914
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Ritz, Anna
中科院分区：
文献类型：
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作者：
Zhuang, Yuan;Cerveny, Kara L.;Ritz, Anna
视黄酸（RA）是维生素A的衍生物，对脊椎动物的生长和发育至关重要。当RA与一般受体（RXR）和RA特异性受体（RAR）的异二聚体结合时，RA调节靶基因的表达，并与称为视黄酸反应元件（RARE）的基序结合[2，3]。对该基序的早期观察鉴定出AGGTCA的完全保守的6聚体直接重复（DR），其由少量核苷酸分开[3]。后来的分析揭示了额外的可变点，导致RARE基序表示为（A/G）G（G/T）T（C/G）A的重复序列，具有1，5或8个核苷酸的间隔区[2]。此外，已知RXR通过该基序与其他结合伴侣如维生素D受体和甲状腺受体相互作用[1]。在这项工作中，我们调查的确切的重复，包括罕见的基序在斑马鱼基因的上游序列的分布。
Retinoic acid (RA) is a derivative of Vitamin A that is essential for vertebrate growth and development. RA regulates the expression of target genes when it binds to a heterodimer of a general receptor (RXR) and an RA-specific receptor (RAR), binding to motifs called Retinoic acid response elements (RAREs)[2, 3]. Early observations of this motif identified a perfectly conserved 6-mer direct repeat (DR) of AGGTCA separated by a small number of nucleotides [3]. Later analysis revealed additional variable points, resulting in a RARE motif represented as a repeat of (A/G) G (G/T) T (C/G) A with a spacer of 1, 5, or 8 nucleotides [2]. Further, RXR is known to interact with other binding partners such as the Vitamin D Receptor and the Thyroid Receptor via this motif [1]. In this work, we investigate the distribution of the exact repeats that comprise the RARE motif in sequences immediately upstream of zebrafish genes.