Histological markers for endothelial cells in endogenous and transplanted rodent pancreatic islets
Histological markers for endothelial cells in endogenous and transplanted rodent pancreatic islets
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DOI：
10.1159/000055906
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发表时间：
2002-01-01
期刊：
影响因子：
3.6
通讯作者：
Jansson, L
中科院分区：
文献类型：
--
作者：
Mattsson, G;Carlsson, PO;Jansson, L
背景/目的：由于内皮细胞的异质性，获得一种用于识别内皮细胞的选择性标记物是困难的。大多数已描述的标记物在某些应用中表现良好，但在其他应用中却不理想。本研究的目的是确定一种用于啮齿类动物微血管内皮细胞的选择性和特异性标记物，尤其用于胰岛血管系统的研究。
方法：将生物素标记的凝集素Bandeiraea或Griffonia simplicifolia与带有碱性磷酸酶的链霉亲和素 - 复合物结合，用于对来自C57BL/6小鼠、Sprague - Dawley大鼠或Wistar - Furth大鼠的石蜡包埋组织切片中的内皮进行染色。
结果：我们能够始终如一地用凝集素Bandeiraea simplicifolia选择性地对肺、小肠、白色和棕色脂肪组织、胰腺以及胰岛中的微血管内皮细胞进行染色。此外，我们能够观察到Wistar - Furth大鼠和C57BL/6小鼠同基因移植胰岛中的血管系统。用抗CD34、CD31、CD200、Ox43、血管性血友病因子以及凝集素Ulex europaeus的抗体对啮齿类动物内皮细胞进行染色的尝试并非都能成功。
结论：凝集素Bandeiraea simplicifolia是一种用于啮齿类动物内皮细胞的通用标记物，可用于研究啮齿类动物胰岛移植后的血管重建。版权所有 (C) 2002 S. Karger AG，巴塞尔和IAP。
Background/Aims:To obtain a selective marker to identify endothelial cells is difficult, due to the heterogeneity of these cells. Most described markers perform well in some applications, but fail in others. The aim of this study was to identify a selective and specific marker for rodent microvascular endothelial cells, especially for use in studies on the vascular system of pancreatic islets. Methods: A biotin-labelled form of the lectin Bandeiraea or Griffonia simplicifolia in combination with a streptAB-Complex with alkaline phosphatase was used to stain endothelium in paraffin-embedded tissue sections from C57BL/6 mice, Sprague-Dawley or Wistar-Furth rats. Results: We were consistently able to selectively stain microvascular endothelial cells in lungs, small intestines, white and brown adipose tissue, pancreas and islets of Langerhans with the lectin Bandeiraea simplicifolia. Furthermore, we were able to visualise the vasculature in syngenically transplanted islets of Langerhans in Wistar-Furth rats and C57BL/6 mice. Attempts to stain rodent endothelial cells with antibodies against CD34, CD31, CD200, Ox43, von Willebrand factor and the lectin Ulex europaeus were not uniformly successful. Conclusion: The lectin Bandeiraea simplicifolia is a versatile marker for rodent endothelial cells, and may be used to study revascularisation after transplantation of pancreatic islet in rodents. Copyright (C) 2002 S. Karger AG, Basel and IAP.