Development of microsatellite markers from the transcriptome of Erysiphe necator for analysing population structure in North America and Europe
Development of microsatellite markers from the transcriptome of Erysiphe necator for analysing population structure in North America and Europe
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DOI：
10.1111/j.1365-3059.2011.02502.x
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发表时间：
2012-02-01
期刊：
影响因子：
2.7
通讯作者：
Milgroom, M. G.
中科院分区：
文献类型：
--
作者：
Frenkel, O.;Portillo, I.;Milgroom, M. G.
利用葡萄白粉病真菌Erysiphe necator的转录组序列，开发微卫星标记（EST-SSRs），研究其在北美东部相对未开发的多样性中心的群体结构。筛选转录组序列发现116个候选微卫星序列，从31个候选微卫星标记中筛选出11个多态性微卫星标记。其中8个标记被用来对来自美国东部不同地区和宿主的分离物进行基因分型，并与来自法国南部和意大利的样本进行比较。美国东部的遗传多样性比欧洲大得多。贝叶斯聚类分析显示，来自北美的10个分离株与欧洲A群亲缘性高，但又不同；这些被称为a样分离株。在美国东部未发现与欧洲B群有密切亲和关系的分离株。贝叶斯分析还发现了圆形叶葡萄的分离株和其他葡萄宿主的分离株之间的遗传分化。美国东北部和东南部的遗传分化主要是由于东南部的a型分离株，其攻击性明显高于其他种群。本研究表明，真菌病原体的转录组测序有助于开发蛋白质编码区域的遗传标记，并强调了这些标记在线虫种群生物学研究中的作用。
Transcriptome sequences of the grape powdery mildew fungus Erysiphe necator were used to develop microsatellite markers (EST-SSRs) to study its relatively unexplored population structure in its centre of diversity in eastern North America. Screening the transcriptome sequences revealed 116 contigs with candidate microsatellites, from which 11 polymorphic microsatellite markers were developed from 31 markers tested. Eight of these markers were used to genotype isolates from different regions and hosts in the eastern USA and compare them to samples from southern France and Italy. Genetic diversity in the eastern USA is much greater than in Europe. Bayesian cluster analyses showed that 10 isolates from North America have high affinities with, but differ from, European group A; these are referred to as A-like isolates. No isolates with close affinity to European group B were found in the eastern USA. Bayesian analyses also detected genetic differentiation between isolates from Vitis rotundifolia and isolates from other Vitis hosts. Genetic differentiation detected between the northeastern and southeastern USA was mostly attributable to the A-like isolates in the southeast, which are significantly more aggressive than the other populations. This research demonstrates that transcriptome sequencing of fungal pathogens is useful for developing genetic markers in protein-coding regions and highlights the role of these markers in population biology studies of E.necator.