Pneumocystis carinii major surface glycoprotein induces interleukin-8 and monocyte chemoattractant protein-I release from a human alveolar epithelial cell line
Pneumocystis carinii major surface glycoprotein induces interleukin-8 and monocyte chemoattractant protein-I release from a human alveolar epithelial cell line
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DOI：
10.1046/j.1365-2362.1999.00517.x
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发表时间：
1999-08-01
影响因子：
5.5
通讯作者：
Lundgren, JD
中科院分区：
文献类型：
--
作者：
Benfield, TL;Lundgren, B;Lundgren, JD
研究背景卡氏肺孢子虫主要表面糖蛋白（MSG）是一种分布于肺孢子虫表面的具有免疫原性的糖蛋白。我们研究了人MSG对肺泡上皮细胞系（A549）分泌趋化因子白细胞介素8（IL-8）和单核细胞趋化蛋白-1（MCF-1）的影响。IL-8释放的依赖性增加（比对照高3.4倍，P < 0.01）。时程实验显示，与对照培养物相比，在4 h、8h和24 h时IL-8释放增加（均P < 0.01）。在24 h时，MCP-1的释放有轻微的（13%）剂量和时间相关性增加（P = 0.02）。MSG与甘露聚糖或β-葡聚糖共孵育分别使IL-8的释放减少48%和42%，表明MSG部分通过碳水化合物部分刺激A549细胞。地塞米松在10（-6）~ 10（-8）mol·L ~（-1）浓度范围内显著抑制MSG诱导的IL-8释放（P < 0.01）。核糖核酸酶保护测定的稳态IL-8 mRNA的增加，在响应味精刺激发生4小时，并持续整个8 h的stimulation.Conclusion这些研究结果表明，味精可以改变肺泡上皮细胞因子的释放，并可能能够调节局部炎症反应，以这种方式。
Background The major surface glycoprotein (MSG) is an abundant, immunogenic glycoprotein located on the surface of Pneumocystis carinii. Little is known about the proinflammatory effects of MSG.Design We have investigated the effect of human MSG on the secretion of the chemokines interleukin 8 (IL-8) and monocyte chemoattractant protein-1 (MCF-1) from an alveolar epithelial cell line (A549).Results Incubation of A549 cells with MSG in concentrations from 0.4 to 10 mu g mL(-1) for 24 h caused dose-dependent increases in IL-8 release (3.4-fold above control, P < 0.01). Time course experiments showed increases in IL-8 release at 4 h, 8 h and 24 h compared with control cultures (all P < 0.01). There was a minor (13%) dose- and time-related increase in MCP-1 release at 24 h (P = 0.02). Co-incubation of MSG with mannan or beta-glucan decreased IL-8 release by 48% and 42% respectively, suggesting that MSG stimulates A549 cells in part through carbohydrate moieties. Dexamethasone significantly inhibited MSG-induced IL-8 release in concentrations of 10(-6)-10(-8) mol L-1 compared with control experiments (P < 0.01). Ribonuclease protection assays for steady-state IL-8 mRNA showed that increases in response to MSG stimulation occurred by 4h and persisted throughout 8 h of stimulation.Conclusion These findings suggest that MSG can alter alveolar epithelial cytokine release and may be capable of modulating the local inflammatory response in this manner.