Silica-coating of fluorescent polystyrene microspheres by a modified Stöber method and their stability against photobleaching
Silica-coating of fluorescent polystyrene microspheres by a modified Stöber method and their stability against photobleaching
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DOI：
10.1515/epoly.2005.5.1.538
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发表时间：
2005-12
期刊：
影响因子：
3.7
通讯作者：
Yoshio Kobayashi;K. Misawa;Masaki Kobayashi;M. Takeda;M. Konno;M. Satake;Y. Kawazoe;N. Ohuchi;A. Kasuya
中科院分区：
文献类型：
--
作者：
Yoshio Kobayashi;K. Misawa;Masaki Kobayashi;M. Takeda;M. Konno;M. Satake;Y. Kawazoe;N. Ohuchi;A. Kasuya
摘要采用基于Stöber方法的种子聚合技术，在亚微米级的聚苯乙烯荧光染料微球(荧光微球)表面包覆了二氧化硅。在0~10g/L聚乙烯吡咯烷酮(PVP)，1~13mol/L水，0~0.8mol/L AQ的存在下，对粒径为100 nm的荧光微球进行了硅胶包覆。氨和0.00038-0.2mol/L正硅酸乙酯(TEOS)。研究发现，PVP的加入抑制了游离二氧化硅颗粒的生成，没有生成无壳荧光微球，提高了硅化荧光微球壳层厚度的均匀性。在10g/L PVP、0.4mol/L AQ时，随着正硅酸乙酯浓度的增加，硅胶壳层厚度从11 nm增加到63 nm。氨水和11摩尔/L水。二氧化硅包覆的荧光微球在激光照射下表现出比未包覆的荧光微球更稳定的荧光。
Abstract Submicron-sized polystyrene spheres incorporating fluorescence dyes (fluorescent microspheres) were coated with silica by means of a seeded polymerization technique based on the Stöber method. Silica-coating of the fluorescent microspheres with a size of 100 nm was performed in the presence of 0 - 10 g/l polyvinylpyrrolidone (PVP), 1 - 13 mol/l water, 0 - 0.8 mol/l aq. ammonia and 0.00038 - 0.2 mol/l tetraethoxyorthosilicate (TEOS). The addition of PVP was found to suppress the generation of free silica particles and produced no shell-free fluorescent microspheres improving the uniformity of shell thickness of silicacoated fluorescent microspheres. Silica shell thickness increased from 11 to 63 nm with increasing TEOS concentration at 10 g/l PVP, 0.4 mol/l aq. ammonia and 11 mol/l water. Silica-coated fluorescent microspheres showed more stable fluorescence to laser-irradiation than uncoated fluorescent microspheres.