SIALOKININ-I AND SIALOKININ-II - VASODILATORY TACHYKININS FROM THE YELLOW-FEVER MOSQUITO AEDES-AEGYPTI
SIALOKININ-I AND SIALOKININ-II - VASODILATORY TACHYKININS FROM THE YELLOW-FEVER MOSQUITO AEDES-AEGYPTI
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DOI：
10.1073/pnas.91.1.138
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发表时间：
1994-01-04
影响因子：
11.1
通讯作者：
RIBEIRO, JMC
中科院分区：
文献类型：
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作者：
CHAMPAGNE, DE;RIBEIRO, JMC
先前有报道称埃及伊蚊的唾液中含有一种分子量为1400道尔顿的肽，具有速激肽典型的药理学特性。在本研究中，这种血管舒张剂被纯化至同质状态，并且发现由两种肽组成：唾液激肽I，其序列为天冬酰胺 - 苏氨酸 - 甘氨酸 - 天冬氨酸 - 赖氨酸 - 苯丙氨酸 - 酪氨酸 - 甘氨酸 - 亮氨酸 - 甲硫氨酸 - 氨，以及唾液激肽II，除了在第1位是天冬氨酸外，与唾液激肽I相同。每对唾液腺中分别含有0.62和0.16皮摩尔（711和178纳克）的这些肽。当在豚鼠回肠上进行测定时，这两种肽与哺乳动物的速激肽P物质活性相同，唾液激肽I、唾液激肽II和P物质的半数有效浓度（K₀.₅）值分别为5.07、6.58和4.94纳摩尔。
The saliva of the mosquito Aedes aegypti has previously been reported to contain a 1400-Da peptide with pharmacological properties typical of a tachykinin. In the present study this vasodilator has been purified to homogeneity- and found to consist of two peptides: sialokinin I, with the sequence Asn-Thr-Gly-Asp-Lys-Phe-Tyr-Gly-Leu-Met-NH2, and sialokinin II, identical to sialokinin I except for an Asp in position 1. These peptides are present in amounts of 0.62 and 0.16 pmol (711 and 178 ng), respectively, per salivary gland pair. When assayed on the guinea pig ileum, both peptides are as active as the mammalian tachykinin substance P, with K0.5 values of 5.07, 6.58, and 4.94 nM for sialokinin I, sialokinin II, and substance P, respectively.