A new sulfated β-galactan from clams with anti-HIV activity
A new sulfated β-galactan from clams with anti-HIV activity
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DOI：
10.1016/s0008-6215(99)00188-3
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发表时间：
1999-09-15
影响因子：
3.1
通讯作者：
Kim, YS
中科院分区：
文献类型：
--
作者：
Amornrut, C;Toida, T;Kim, YS
从海洋蛤属物种文蛤中分离出一种由具有β-（1-> 3）-糖苷键的硫酸半乳聚糖组成的新多糖。该多糖是均匀的，其组成中含有D-半乳糖。通过2D DQF-COSY和2D NOESY光谱检查糖苷键。异头质子的耦合常数为7.8 Hz，表明为β-半乳糖构型。半乳糖残基H-2的低场位移证明了2-O-磺酸基的存在。TQF-COSY证实C-6位被磺酸酯基团取代。通过抑制合胞体形成来评价多糖的抗HIV活性。硫酸化半乳聚糖的融合指数和融合抑制百分比在200 μ g/mL时分别为0.34和56%。(C)1999 Elsevier Science Ltd.保留所有权利。
A new polysaccharide composed of galactan sulfate with a beta-(l --> 3)-glycosidic linkage has been isolated from the marine clam species Meretrix petechialis. The polysaccharide was homogeneous in its composition containing D-galactose. The glycosidic linkage was examined by 2D DQF-COSY and 2D NOESY spectroscopy. The coupling constant of anomeric proton was 7.8 Hz, suggesting a beta-galacto configuration. The downfield shift of H-2 of galactose residue demonstrated the presence of 2-O-sulfonate group. TQF-COSY confirmed that the C-6 position was substituted with a sulfonate group. The anti-HIV activity of the polysaccharides has been evaluated by the inhibition of syncytia formation. The fusion index and percentage fusion inhibition of sulfated galactan were 0.34 and 56% at 200 mu g/mL. (C) 1999 Elsevier Science Ltd. All rights reserved.