Emerging applications of flow cytometry in solid tumor biology
Emerging applications of flow cytometry in solid tumor biology
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DOI：
10.1016/j.ymeth.2012.03.027
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发表时间：
2012-07-01
期刊：
影响因子：
4.8
通讯作者：
Hedley, David
中科院分区：
文献类型：
--
作者：
Chang, Qing;Hedley, David
尽管在流式细胞术的早期临床开发过程中引起了相当大的兴趣，但近年来其在实体瘤中的应用在很大程度上被忽视了。然而，随着癌症生物学和分子治疗学的快速发展，以及流式细胞仪仪器、试剂和数据分析领域的技术发展，重新评估这一作用是及时的。本文强调了流式细胞术分析异质细胞群的独特潜力，并通过同时测量多个关键要素提供有关调控过程功能状态的信息。进展的主要障碍包括获得足够的临床样品，组织解聚产生适合流式细胞术的单细胞悬浮液，以及标记细胞内和细胞表面抗原的协议。(C)2012 Elsevier Inc. All rights reserved.
Despite considerable interest during the early clinical development of flow cytometry, its application to solid tumours has been largely ignored in recent years. However, with rapid progress in cancer biology and molecular therapeutics, linked to technical developments in the areas of flow cytometry instrumentation, reagents, and data analysis, it is timely to re-evaluate this role. This article places emphasis on the unique potential of flow cytometry to analyze heterogeneous cell populations, and to provide information on the functional status of regulatory processes by the simultaneous measurement of multiple key elements. Major obstacles to progress addressed include the acquisition of adequate clinical samples, tissue disaggregation to produce single cells suspensions suitable for flow cytometry, and protocols to label intracellular as well as cell surface antigens. (C) 2012 Elsevier Inc. All rights reserved.