Endogenous electric fields in embryos during development, regeneration and wound healing
Endogenous electric fields in embryos during development, regeneration and wound healing
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DOI：
10.1093/oxfordjournals.rpd.a006375
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发表时间：
2003-01-01
影响因子：
1
通讯作者：
Nuccitelli, R
中科院分区：
文献类型：
--
作者：
Nuccitelli, R
所有被研究的胚胎都驱动离子电流通过它们自己，这些电流将产生内部电场。在这里，这些领域已经被直接测量的例子进行了讨论。第一次这样的测量是在鸡胚胎中进行的，在2-4天的胚胎中，在后肠门附近测量到约20 mV mm（-1）。这个电场对于尾部结构的发展是重要的，因为降低其强度会导致异常的尾部发展。直接测量的第二个胚胎电场是在蝾螈体内，在那里检测到了大约相同大小的吻尾电场。在神经胚形成而不是原肠胚形成期间，该领域的修改引起发育异常。最近，在青蛙和鸡胚胎中发现左右不对称的发展需要在非常早期的发育阶段卵裂球之间的电压差。在鸡胚中测得该电场为10-20 mV min（-1），穿过原始条纹。哺乳动物皮肤伤口在伤口侧面产生150 mV mm（-1）场，角膜表皮伤口表现出40 mV mm（-1）的横向场。这些内源性电场的存在表明，暴露于外部电场应限于小于0.1 V m（-1）的幅度。
All embryos that have been investigated drive ionic currents through themselves and these currents will generate internal electric fields. Here, those examples in which such fields have been measured directly are discussed. The first such measurements were made in chick embryos and about 20 mV mm(-1) was measured near the posterior intestinal portal in 2-4-day-old embryos. This electric field is important for the development of tail structures because reducing its magnitude results in abnormal tail development. The second embryonic electric field measured directly was in the axolotl, where a rostral-caudal field of about the same magnitude was detected. Modification of this field during neurulation but not gastrulation caused developmental abnormalities. Most recently, the development of left-right asymmetry in frog and chick embryos was found to require a voltage difference between blastomeres at a very early developmental stage. This field was measured in the chick embryo to be 10-20 mV min(-1) across the primitive streak. Mammalian skin wounds generate 150 mV mm(-1) fields lateral to the wound and corneal epidermal wounds exhibit lateral fields of 40 mV mm(-1). The presence of these endogenous fields would suggest that exposures to external electric fields should be limited to magnitudes of less than 0.1 V m(-1).