The Mechanism of Rap1 Regulates N-cadherin to Control Neuronal Migration
The Mechanism of Rap1 Regulates N-cadherin to Control Neuronal Migration
复制标题
Rap1调节N-钙粘蛋白控制神经元迁移的机制
DOI：
10.1007/s12031-019-01316-w
复制
发表时间：
2019
影响因子：
3.1
通讯作者：
Lin Juntang
中科院分区：
文献类型：
--
作者：
Yang Ciqing;Li Xiaoying;Zhang Bichao;Fu Sulei;Li Shuanqing;Shen Jianing;Guan Lihong;Qiao Liang;Lin Juntang
Rap 1和N-cadherin调节皮层神经元的非胶质细胞依赖性易位。Rap 1是如何调节N-钙粘蛋白介导的神经元迁移的尚不清楚。在这里，我们在小鼠大脑（胚胎第16天）中过表达Rap 1gap以表达Rap 1，并观察到神经元没有迁移到外层。我们证实Rap 1参与了体内晚期神经元的调节。在CHO细胞中Rap 1gap过表达和Rap 1抑制降低了细胞骨架蛋白如微管蛋白的表达。还观察到细胞形态调节因子如N-钙粘蛋白和β-连环蛋白表达的变化。抑制小鼠脑中的N-钙粘蛋白阻止神经元迁移到外层。Rap 1gap过表达后CHO细胞形态发生改变。我们认为Rap 1在胚胎发育过程中调节N-cadherin的表达，进而影响β-catenin的表达。β-连环蛋白反过来调节细胞骨架蛋白的表达，最终影响神经元的形态和迁移。
Rap1 and N-cadherin regulate glia-independent translocation of cortical neurons. It remains unclear how Rap1 regulates N-cadherin-mediated neuronal migration. Here, we overexpressed Rap1gap in mouse brains (embryonic day 16) to inactivate Rap1, and observed that neurons did not migrate to the outer layer. We confirmed that Rap1 was involved in the regulation of late neurons in vivo. Rap1gap overexpression and Rap1 suppression in CHO cells decreased the expression of cytoskeletal proteins such as tubulin. Changes in the expression of cell morphology regulators, such as N-cadherin and β-catenin, were also observed. Inhibition of N-cadherin in mouse brains prevented neuronal migration to the outer layer. The morphology of CHO cells was changed after overexpression of Rap1gap. We propose that Rap1 regulates the expression of N-cadherin during embryonic development, which affects β-catenin expression. Beta-catenin in turn regulates cytoskeletal protein expression, ultimately affecting neuronal morphology and migration.