Influence of systematically varied nanoscale topography on the morphology of epithelial cells
Influence of systematically varied nanoscale topography on the morphology of epithelial cells
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DOI：
10.1109/tnb.2003.813934
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发表时间：
2003-06-01
影响因子：
3.9
通讯作者：
Sutherland, DS
中科院分区：
文献类型：
--
作者：
Andersson, AS;Brink, J;Sutherland, DS
制造纳米尺寸的表面物体，我们的兴趣关注细胞是否会响应系统增加尺寸的表面结构。我们的方法来回答这个问题tematically是制造表面相同的表面化学和类似的表面粗糙度，但增加的结构特征的大小。为了制造细胞培养研究所需的大面积图案化表面，我们使用胶体光刻法，利用胶体颗粒作为表面纳米结构化的模板。所制造的表面含有直径范围为60至170 nm的半球形纳米柱。通过评价细胞面积和细胞形状来研究胰腺上皮细胞系（AR 4 - 2 J）的细胞形态变化。后者研究了应用细胞形状分类方法，使用三个形状描述符。胰腺细胞以系统的方式对表面纳米结构做出反应。当在具有增大的地形特征的表面上培养时，细胞扩散得更多并且变得更加非圆形。
manufactured nanometer-sized surface objects, our interest concerned whether cells would respond to surface structures of sys- increasing size. Our approach to answer this question tematically was to fabricate surfaces with the same surface chemistry and similar surface roughness but increasing size of structural features. To fabricate large areas of patterned surfaces, required for cell culture studies, we used colloidal lithography utilizing colloidal particles as a template for surface nanostructuring. The fabricated surfaces contained hemispherical nanopillars with diameters ranging from 60 to 170 nm. Changes in cell morphology of a pancreatic epithelial cell line (AR4-2J) were studied by evaluating cell area and cell shape. The latter was studied by applying the cell shape classification method using three shape descriptors. The pancreatic cells responded in a systematic way to the surface nanostructures. The cells spread more and became more nonround when cultured on surfaces with increasing size of the topographic features.