An Evaluation of Protein Intake for Metabolic Demands and the Quality of Dietary Protein in Rats Using an Indicator Amino Acid Oxidation Method
An Evaluation of Protein Intake for Metabolic Demands and the Quality of Dietary Protein in Rats Using an Indicator Amino Acid Oxidation Method
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DOI：
10.3177/jnsv.57.418
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发表时间：
2011-12-01
影响因子：
1.6
通讯作者：
Kido, Yasuhiro
中科院分区：
文献类型：
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作者：
Ogawa, Aki;Naruse, Yuka;Kido, Yasuhiro
目前。蛋白质需要量通常是根据氮平衡研究确定的，但这种方法有许多局限性。指示剂氨基酸氧化（IAAO）方法的理论基础与氮平衡方法有很大不同，是作为人类的替代方法开发的。本研究的目的是评估蛋白质摄入量的代谢需求和蛋白质的质量，使用蛋白质本身，在大鼠中采用IAAO技术与L，[1-C-13]苯丙氨酸。雄性Wistar/ST大鼠（5-6周龄）接受分级酪蛋白（4.3、8.6、12.9、17.2. 21.5，25.8%）或小麦面筋（7.2，10.8，14.4，18.0，21.6. 25.2%），沿着添加L-[1-C-13]苯丙氨酸。在C-13摄入开始后210分钟，呼吸中达到同位素平台。通过将混合效应变点回归模型应用于呼吸（CO2）-C-13数据来计算代谢需求的蛋白质摄入量，该模型确定了响应分级蛋白质摄入量的最小呼吸（CO2）-C-13处的断点。用IAAO法测定的蛋白质代谢需要量为酪蛋白13.1 g/kg BW/d和谷朊粉18.1 g/kg BW/d。显示出与通过氮平衡法测定的趋势相似的趋势。这些结果表明，IAAO方法不仅可用于评估代谢需求的蛋白质摄入量，而且可用于评估自由生活大鼠的膳食蛋白质质量，这表明该方法在临床环境中可能是可行的。
Currently. protein requirements are generally determined based on nitrogen balance studies, but there are a variety of limitations associated with this method. The indicator amino acid oxidation (IAAO) method, with a theoretical base that differs widely from the nitrogen balance method, was developed as an alternative method for humans. The objective of the present study was to evaluate protein intakes for metabolic demands and protein quality, using protein itself, in rats employing the IAAO technique with L,[1-C-13]phenylalanine. Male Wistar/ST rats (5-6 wk old) received a graded casein (4.3, 8.6, 12.9, 17.2. 21.5, 25.8%), or a wheat gluten (7.2, 10.8, 14.4, 18.0, 21.6. 25.2%) diet, along with L-[1-C-13]phenylalanine. An isotopic plateau in breath was achieved 210 min after the start of the C-13 ingestion. The protein intakes for metabolic demands were calculated by applying a mixed-effect change-point regression model to breath (CO2)-C-13 data, which identified a breakpoint at minimal breath (CO2)-C-13 in response to graded protein intake. The protein intakes for metabolic demands determined by the IAAO method were 13.1 g/kg BW/d for casein and 18.1 g/kg BW/d for wheat gluten. showing a tendency similar to that determined by the nitrogen balance method. These results demonstrated that the IAAO method could be employed to evaluate not only the protein intakes for metabolic demands, but the dietary protein quality in freely living rats, suggesting that this method might be viable in a clinical setting.