Role of macrophages in depot-dependent browning of white adipose tissue
Role of macrophages in depot-dependent browning of white adipose tissue
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DOI：
10.1007/s12576-017-0567-3
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发表时间：
2018-09-01
影响因子：
2.3
通讯作者：
Kimura, Kazuhiro
中科院分区：
文献类型：
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作者：
Machida, Ken;Okamatsu-Ogura, Yuko;Kimura, Kazuhiro
交感神经刺激会诱导白色脂肪组织 (WAT) 中的米色脂肪细胞，称为 WAT 褐变。在这项研究中，将小鼠暴露在寒冷环境温度（10°C）下 24 小时，可诱导腹股沟 WAT 中解偶联蛋白 1 (UCP1)（米色脂肪细胞标记物）的 mRNA 表达，但在性腺周围 WAT 中则不然。因此，我们研究了巨噬细胞在小鼠体内依赖于仓库的 WAT 褐变中的作用。流式细胞术分析表明，性腺周围WAT中的巨噬细胞总数高于腹股沟WAT中的巨噬细胞总数。冷暴露未能改变腹股沟WAT中巨噬细胞标记基因的表达；然而，它增加了性腺周围WAT中CD11c和肿瘤坏死因子-α的mRNA表达，表明促炎M1巨噬细胞被激活。使用氯膦酸盐去除巨噬细胞显着增强了性腺周围 WAT 中冷诱导的 UCP1 mRNA 表达。这些结果表明，M1 巨噬细胞参与了几乎不发生褐变的性腺周围 WAT 的表型。
Sympathetic stimulation induces beige adipocytes in white adipose tissue (WAT), known as browning of WAT. In this study, exposure of mice to cold ambient temperature (10 A degrees C) for 24 h induced the mRNA expression of uncoupling protein 1 (UCP1), a marker for beige adipocytes, in inguinal WAT, but not in perigonadal WAT. Thus, we examined the role of macrophages in depot-dependent WAT browning in mice. Flowcytometric analysis showed that total number of macrophages was higher in perigonadal WAT than in inguinal WAT. Cold exposure failed to change the expression of macrophage marker genes in inguinal WAT; however, it increased the mRNA expression of CD11c and tumor necrosis factor-alpha in perigonadal WAT, indicating that proinflammatory M1 macrophage is activated. The removal of macrophages using clodronate significantly enhanced cold-induced UCP1 mRNA expression in perigonadal WAT. These results suggest that M1 macrophages are involved in the phenotype of perigonadal WAT that hardly undergo browning.