Rat anterior pituitary cells in vitro can partly reconstruct in vivo topographic affinities
Rat anterior pituitary cells in vitro can partly reconstruct in vivo topographic affinities
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DOI：
10.1002/ar.a.10065
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发表时间：
2003-06-01
期刊：
影响因子：
--
通讯作者：
Yashiro, T
中科院分区：
文献类型：
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作者：
Noda, T;Kikuchi, M;Yashiro, T
大鼠垂体前叶中产生激素的细胞不是随机分布的；相反，五种细胞类型之间存在特定的拓扑亲和力（Noda 等人，Acta Histochem. Cytochem. 200.1；34：313-319）。在这项研究中，我们至少部分地在原代单层培养物中重建了这些亲和力。从成年雄性大鼠收集的垂体细胞经酶分散并以1 x 10(5)细胞/cm(2)的密度培养72小时。我们使用抗激素抗体对细胞进行双重免疫染色，然后使用共聚焦激光显微镜检查细胞彼此附着的能力。我们还统计分析了五种激素产生细胞的所有组合的亲和力。我们通过电子显微镜观察簇以识别细胞之间的连接复合物。共聚焦激光显微镜表明体内激素产生细胞的特征和附着模式与体外相似。统计分析显示，五种激素生成细胞与生长激素（GH）、催乳素（PAL）和黄体生成素（LH）生成细胞的附着率不相等，这表明存在特定的拓扑亲和力。促肾上腺皮质激素（ACTH）产生细胞与GH细胞、LH与PRL细胞以及PRL与LH细胞的附着率较高，而PRL与PRL细胞的附着率较低。此外，该比率与我们之前的体内研究数据相关。超微结构观察显示激素产生细胞之间几乎没有连接复合物。这些结果表明，垂体前叶激素产生细胞可以通过特异性和/或非特异性粘附因子附着到特定类型的细胞，并且可以重建垂体的拓扑性质。 Anat Rec Part A 272A:548-555, 2003。(C) 2003 Wiley-Liss, Inc.
Hormone-producing cells in the rat anterior pituitary gland are not randomly distributed; rather, there are specific topographic affinities among five cell types (Noda et al., Acta Histochem. Cytochem. 200.1;34:313-319). In this study we reconstructed these affinities, at least partially, in primary monolayer culture. Pituitary cells collected from adult male rats were enzymatically dispersed and cultured for 72 hr at a density of 1 x 10(5) cells/cm(2). We double-immunostained cells using antibodies against hormones, and then used confocal laser microscopy to examine the ability of the cells to attach to each other. We also statistically analyzed the affinity of all combinations of the five types of hormone-producing cells. We observed clusters by electron microscopy to identify junctional complexes between the cells. Confocal laser microscopy indicated that the features and attachment patterns of hormone-producing cells in vivo were similar to those in vitro. Statistical analyses revealed that the rates at which the five types of hormone-producing cells attached to growth hormone (GH)-, prolactin (PAL), and luteinizing hormone (LH)-producing cells were unequal, which suggests there are specific topographic affinities. The specific rates of adrenocorticotropic hormone (ACTH)-producing cell attachment to GH cells, LH to PRL cells, and PRL to LH cells were high, whereas that of PRL attachment to PRL cells was low. In addition, the rates correlated with the data from our previous in vivo study. Ultrastructural observations revealed few junctional complexes between hormone-producing cells. These results indicate that anterior pituitary hormone-producing cells can attach to specific types of cells by means of specific and/or nonspecific adhesion factors, and can reconstruct the topographic nature of the pituitary gland. Anat Rec Part A 272A:548-555, 2003. (C) 2003 Wiley-Liss, Inc.