Mass spectrometry in viral proteomics.
Mass spectrometry in viral proteomics.
复制标题
病毒蛋白质组学中的质谱分析。
DOI：
10.1021/ar9801200
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Siuzdak,G
中科院分区：
文献类型：
--
作者：
Thomas,JJ;Bakhtiar,R;Siuzdak,G
在结构和功能病毒蛋白质组学中，质谱学是一种有价值的工具，它已被用于鉴定病毒衣壳蛋白、病毒突变和翻译后修饰。此外，基于质量的方法与时间分辨蛋白分解(质量图谱)相结合，揭示了溶液中病毒颗粒的动态性质；该方法有助于了解病毒衣壳的动态结构域，这可能对开发新的病毒灭活方法具有重要价值。作为这些实验的结果，通过与来自X射线结晶学的补充数据进行比较，病毒蛋白质结构和功能的新维度正在出现。
Mass spectrometry is a valuable tool in structural and functional viral proteomics, where it has been used to identify viral capsid proteins, viral mutants, and posttranslational modifications. Further, mass-based approaches combined with time-resolved proteolysis (mass mapping) have revealed the dynamic nature of viral particles in solution; this method is contributing to an understanding of the dynamic domains of the viral capsid which may have significant value in developing new approaches for viral inactivation. As a result of these experiments, and by comparison with complementary data from X-ray crystallography, a new dimension to viral protein structure and function is emerging.