Short communication: Molecular typing of Prototheca zopfii from bovine mastitis in Japan
Short communication: Molecular typing of Prototheca zopfii from bovine mastitis in Japan
复制标题
DOI：
10.3168/jds.2011-5101
复制
发表时间：
2012-08-01
影响因子：
3.5
通讯作者：
Kamata, H.
中科院分区：
文献类型：
--
作者：
Sobukawa, H.;Yamaguchi, S.;Kamata, H.
佐氏无绿藻引起牛乳腺炎，导致产奶量减少和分泌带有白色薄片的稀牛奶。佐氏无绿藻（Prototheca zopfii）在生物化学和血清学上被分为至少2种基因型，佐氏无绿藻基因型1和佐氏无绿藻基因型2。已知后者是牛原鞘乳腺炎的主要病原体。Prototheca zopfii后来被重新分类为5个变种：var. zopfii（基因型1和2），var. 1（原Prototheca blaschkeae），var. 3（formerly P. moriformis），and var. portoricensis。在这项研究中，18S核糖体DNA序列的不同临床标本，从不同地区在日本进行了研究，以阐明致病性的P. zopfii变种。zopfii。系统发育树显示，所有基因型2的菌株聚在一个聚簇的P. zopfii var。Zopfii SAG 2021（T）（模式株基因型2），与基因型1分离株群无关。因此，所有来自日本牛乳腺炎的分离株均被鉴定为佐氏疟原虫基因型2。因此，P. zopfii var. Zopfii基因型2与牛乳腺炎相关。
Prototheca zopfii causes bovine mastitis, resulting in reduced milk production and the secretion of thin watery milk with white flakes. Prototheca zopfii has been biochemically and serologically divided into at least 2 genotypes, P. zopfii genotype 1 and P. zopfii genotype 2. The latter is known to be the main causative agent of bovine protothecal mastitis. Prototheca zopfii was later reclassified into 5 varieties: var. zopfii (genotypes 1 and 2), var. 1 (formerly Prototheca blaschkeae), var. 3 (formerly P. moriformis), and var. portoricensis. In this study, the 18S ribosomal DNA sequences of diverse clinical specimens from different areas in Japan were studied to clarify the pathogenicity of P. zopfii var. zopfii. The phylogenetic tree revealed that all genotype 2 isolates were grouped in a cluster of P. zopfii var. zopfii SAG 2021(T) (type strain genotype 2), and were independent from the cluster of the genotype 1 isolates. Thus, all isolates from bovine mastitis in Japan were identified as P. zopfii genotype 2. Therefore, P. zopfii var. zopfii genotype 2 is associated with bovine mastitis.