A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
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DOI：
10.26508/lsa.202000714
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发表时间：
2020-09-01
影响因子：
4.4
通讯作者：
Bethune, Julien
中科院分区：
文献类型：
--
作者：
Goyal, Manu Jain;Zhao, Xiyan;Bethune, Julien
外壳蛋白复合体I （COPI）包被的囊泡介导高尔基池之间的膜运输以及蛋白质从高尔基体到内质网的回收。由蛋白质复合体涂层的相似亚基定义的COPI涂层有几种风味。然而，目前尚不清楚类似的COPI蛋白是否具有特定的功能。本研究表明，在小鼠多能性细胞的神经元分化过程中，同源涂层亚基γ - 1-COP和γ - 2-COP的表达存在差异。此外，通过基因组编辑实验的组合，我们证明了尽管γ - cop相似物在很大程度上是功能冗余的，但γ - 1-COP特异性地促进了神经突的生长。我们的工作强调了COPI通路在神经元极化中的作用，并为涂层旁系亚基在这一过程中的不同功能提供了证据。
Coat protein complex I (COPI)-coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits gamma 1-COP and gamma 2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas gamma-COP paralogs are largely functionally redundant, gamma 1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process.