Inducible expression of neuronal glutamate receptor channels in the NT2 human cell line.
Inducible expression of neuronal glutamate receptor channels in the NT2 human cell line.
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DOI：
10.1073/pnas.90.6.2174
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发表时间：
1993-03
影响因子：
11.1
通讯作者：
D. P. Younkin;Cha Min Tang;M. Hardy;Usha Rani Reddy;Qing Yao Shi;Samuel J. Pleasure;Virginia M.-Y. Lee;David E Pleasure
中科院分区：
文献类型：
--
作者：
D. P. Younkin;Cha Min Tang;M. Hardy;Usha Rani Reddy;Qing Yao Shi;Samuel J. Pleasure;Virginia M.-Y. Lee;David E Pleasure
谷氨酸受体（GluR）通道负责哺乳动物中枢神经系统的许多基本性质，包括几乎所有的兴奋性突触传递、突触可塑性和兴奋性毒素介导的神经元死亡。虽然许多人类和啮齿动物的神经母细胞系是可用的，没有直接显示出表达GluR通道。我们在这里报告，细胞从人畸胎瘤系NT 2诱导维甲酸表达神经元N-甲基-D-天冬氨酸（NMDA）和非NMDA GluR通道伴随着他们的终末分化成神经元样细胞。这些人GluR通道的分子和生理特征与中枢神经系统神经元中的分子和生理特征几乎相同，如PCR和膜片钳记录所示，并且细胞表现出谷氨酸诱导的神经毒性。
Glutamate receptor (GluR) channels are responsible for a number of fundamental properties of the mammalian central nervous system, including nearly all excitatory synaptic transmission, synaptic plasticity, and excitotoxin-mediated neuronal death. Although many human and rodent neuroblast cell lines are available, none has been directly shown to express GluR channels. We report here that cells from the human teratocarcinoma line NT2 are induced by retinoic acid to express neuronal N-methyl-D-aspartate (NMDA) and non-NMDA GluR channels concomitant with their terminal differentiation into neuron-like cells. The molecular and physiologic characteristics of these human GluR channels are nearly identical to those in central nervous system neurons, as demonstrated by PCR and patch clamp recordings, and the cells demonstrate glutamate-induced neurotoxicity.