Self-assembly of thermo-reversible hydrogels via molecular recognition toward a spatially organized co-culture system
Self-assembly of thermo-reversible hydrogels via molecular recognition toward a spatially organized co-culture system
复制标题
通过分子识别将热可逆水凝胶自组装成空间组织的共培养系统
DOI：
10.1021/acs.biomac.6b01672
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发表时间：
2017
影响因子：
6.2
通讯作者：
Ryo Yoshida
中科院分区：
文献类型：
--
作者：
Ryota Tamate;Kotomi Takahashi;Takeshi Ueki;Aya Mizutani Akimoto;Ryo Yoshida
在这项研究中，我们提出了两种热可逆的物理交联水凝胶在生理条件下通过分子识别的自发粘附。我们成功地用两种定义良好的ABA型三嵌段共聚物制备了两种水凝胶：CAT-ABA和PBA-ABA，它们分别含有儿茶酚和苯硼酸基团作为功能侧链。两种类型的ABA三嵌段共聚物都能够随着温度的升高而发生溶胶到凝胶的转变，这是由于在生理温度下形成物理交联，这使得细胞在水凝胶中很容易被包封。结果表明，包封细胞的水凝胶通过在水凝胶界面苯基硼酸和儿茶酚之间形成硼酯，表现出自发的宏观粘附。这种新系统可能代表了一种很有前途的方法，可以构建精确组织的三维共培养系统，从而能够在体外重建复杂的组织，如肝脏。
In this study, we present the spontaneous adhesion of two thermoreversible physically cross-linked hydrogels via molecular recognition under a physiological condition. We successfully prepared two types of hydrogels generated using two kinds of well-defined ABA type triblock copolymers: CAT-ABA and PBA-ABA, which contain catechol and phenylboronic acid groups as functional side chains, respectively. Both types of ABA triblock copolymers were able to undergo sol-to-gel transition with the increase in temperature resulting from the formation of physical cross-links at a physiological temperature, which enables easy cell encapsulation in the hydrogel. It was determined that the cell encapsulating hydrogels exhibited spontaneous macroscopic adhesion through the formation of boronic esters between phenylboronic acid and catechol at the hydrogel interface. This novel system likely represents a promising method to construct a precisely organized, three-dimensional coculture system to enable the reconstruction of complicated tissues such as the liver in vitro.