A Seven-Transmembrane Receptor That Mediates Avoidance Response to Dihydrocaffeic Acid, a Water-Soluble Repellent in Caenorhabditis elegans
A Seven-Transmembrane Receptor That Mediates Avoidance Response to Dihydrocaffeic Acid, a Water-Soluble Repellent in Caenorhabditis elegans
复制标题
DOI：
10.1523/jneurosci.4018-11.2011
复制
发表时间：
2011-11-16
影响因子：
5.3
通讯作者：
Goshima, Yoshio
中科院分区：
文献类型：
--
作者：
Aoki, Reina;Yagami, Tatsurou;Goshima, Yoshio
快速检测有害化学物质的能力对所有动物的生存至关重要。在秀丽隐杆线虫（C. habditis elegans）中，elegans），驱避剂触发回避反应，导致动物远离驱避剂。二氢咖啡酸（DHCA）是一种水溶性驱虫剂和非黄酮类儿茶酚化合物，可在植物产品中发现。以非洲爪蟾Xenopus laevis（X. laevis）卵母细胞表达系统中，我们鉴定了一个候选的二氢咖啡酸受体（DCAR），DCAR-1。DCAR-1是一种新的七跨膜蛋白，表达于C.优雅的。dcar-1突变动物在对DHCA的回避反应中有缺陷，并且dcar-1突变动物的ASH神经元中dcar-1的细胞特异性表达挽救了对DHCA的回避反应中的缺陷。我们的研究结果确定DCAR-1作为第一个七跨膜受体所需的避免水溶性驱避剂，DHCA，在C。优美的
The ability to detect harmful chemicals rapidly is essential for the survival of all animals. In Caenorhabditis elegans (C. elegans), repellents trigger an avoidance response, causing animals to move away from repellents. Dihydrocaffeic acid (DHCA) is a water-soluble repellent and nonflavonoid catecholic compound that can be found in plant products. Using a Xenopus laevis (X. laevis) oocyte expression system, we identified a candidate dihydrocaffeic acid receptor (DCAR), DCAR-1. DCAR-1 is a novel seven-transmembrane protein that is expressed in the ASH avoidance sensory neurons of C. elegans. dcar-1 mutant animals are defective in avoidance response to DHCA, and cell-specific expression of dcar-1 in the ASH neurons of dcar-1 mutant animals rescued the defect in avoidance response to DHCA. Our findings identify DCAR-1 as the first seven-transmembrane receptor required for avoidance of a water-soluble repellent, DHCA, in C. elegans.