Effects of Arabinoxylan and Resistant Starch on Intestinal Microbiota and Short-Chain Fatty Acids in Subjects with Metabolic Syndrome: A Randomised Crossover Study.
Effects of Arabinoxylan and Resistant Starch on Intestinal Microbiota and Short-Chain Fatty Acids in Subjects with Metabolic Syndrome: A Randomised Crossover Study.
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DOI：
10.1371/journal.pone.0159223
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Dahlerup JF
中科院分区：
文献类型：
--
作者：
Hald S;Schioldan AG;Moore ME;Dige A;Lærke HN;Agnholt J;Bach Knudsen KE;Hermansen K;Marco ML;Gregersen S;Dahlerup JF
最近，肠道微生物群被强调为代谢综合征发展的重要贡献者。膳食纤维可通过调节肠道微生物群和代谢终产物发挥有益作用。我们研究了富含两种不同膳食纤维（阿拉伯木聚糖和2型抗性淀粉）的饮食对肠道微生物组和粪便短链脂肪酸的影响。19名患有代谢综合征的成年人完成了这项随机交叉研究，进行了两项为期4周的干预，即富含阿拉伯木聚糖和抗性淀粉的饮食和低纤维西式饮食。在干预前和干预结束时收集粪便样品，进行发酵终产物分析和16S核糖体RNA细菌基因扩增，以鉴定细菌分类群。粪便碳水化合物残留用于验证依从性。富含阿拉伯木聚糖和抗性淀粉的饮食导致粪便相关肠道微生物群的总物种多样性显著降低，但也增加了受试者之间和受试者内细菌群落的异质性。阿拉伯木聚糖和抗性淀粉的摄入增加了双歧杆菌的比例（P<0.001），而与肠道生态失调相关的某些细菌属的比例则降低了。此外，总短链脂肪酸（P<0.01），乙酸（P<0.01）和丁酸（P<0.01）的浓度高，结束时的饮食富含阿拉伯木聚糖和抗性淀粉相比，从西式饮食。异丁酸（P = 0.05）和异戊酸（P = 0.03）的浓度响应于富含阿拉伯木聚糖和抗性淀粉的饮食而降低，表明蛋白质发酵减少。总之，阿拉伯木聚糖和抗性淀粉的摄入改变了微生物组和短链脂肪酸组成，对结肠健康和代谢综合征具有潜在的有益影响。ClinicalTrials.gov www.example.com
Recently, the intestinal microbiota has been emphasised as an important contributor to the development of metabolic syndrome. Dietary fibre may exert beneficial effects through modulation of the intestinal microbiota and metabolic end products. We investigated the effects of a diet enriched with two different dietary fibres, arabinoxylan and resistant starch type 2, on the gut microbiome and faecal short-chain fatty acids. Nineteen adults with metabolic syndrome completed this randomised crossover study with two 4-week interventions of a diet enriched with arabinoxylan and resistant starch and a low-fibre Western-style diet. Faecal samples were collected before and at the end of the interventions for fermentative end-product analysis and 16S ribosomal RNA bacterial gene amplification for identification of bacterial taxa. Faecal carbohydrate residues were used to verify compliance. The diet enriched with arabinoxylan and resistant starch resulted in significant reductions in the total species diversity of the faecal-associated intestinal microbiota but also increased the heterogeneity of bacterial communities both between and within subjects. The proportion of Bifidobacterium was increased by arabinoxylan and resistant starch consumption (P<0.001), whereas the proportions of certain bacterial genera associated with dysbiotic intestinal communities were reduced. Furthermore, the total short-chain fatty acids (P<0.01), acetate (P<0.01) and butyrate concentrations (P<0.01) were higher by the end of the diet enriched with arabinoxylan and resistant starch compared with those resulting from the Western-style diet. The concentrations of isobutyrate (P = 0.05) and isovalerate (P = 0.03) decreased in response to the arabinoxylan and resistant starch enriched diet, indicating reduced protein fermentation. In conclusion, arabinoxylan and resistant starch intake changes the microbiome and short-chain fatty acid compositions, with potential beneficial effects on colonic health and metabolic syndrome. ClinicalTrials.gov NCT01618526