STUDIES ON L-ARGINASE IN DEVELOPING RAT SMALL-INTESTINE, BRAIN, AND KIDNEY .1. ONTOGENIC EVOLUTION OF ARGINASE ISOENZYMES
STUDIES ON L-ARGINASE IN DEVELOPING RAT SMALL-INTESTINE, BRAIN, AND KIDNEY .1. ONTOGENIC EVOLUTION OF ARGINASE ISOENZYMES
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DOI：
10.1016/0885-4505(86)90070-8
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发表时间：
1986-04-01
期刊：
影响因子：
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通讯作者：
TOMASZEWSKI, L
中科院分区：
文献类型：
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作者：
KONARSKA, L;TOMASZEWSKI, L
大鼠肠、肾和脑中精氨酸酶同工酶的成体模式几乎相同，由阳离子A1和阴离子A4两种形式组成。本文报道了这些组织中酶设备的器官特异性成熟。各组织中精氨酸酶活性在妊娠第13~16天均可检测到。在胎儿的肠和肾中，精氨酸酶活性较低，并持续到断奶期间，酶活性迅速上升，达到10倍。然而，这些组织中的精氨酸酶同工酶的成体模式是以不同的方式完成的。在肠道中，精氨酸酶A1出现在胎儿生命中，是这种酶的唯一形式，直到出生后第19天到第21天，第二种形式的精氨酸酶A4出现并迅速积累，这是断奶时总酶活性上升的唯一原因。在肾脏中，精氨酸酶A1单独存在于胎儿早期。精氨酸酶A4在出生前3-4天出现，其活性在出生后的头两周内保持不变。断奶时肾脏精氨酸酶总比活力的显著升高是由于先前存在的精氨酸酶A4的积累。在大脑中，精氨酸酶同工酶的成体模式比其他组织更早实现。这两种形式，A1和A4，都发生在怀孕13-14天。
The adult patterns of arginase isoenzymes in rat intestine, kidney, and brain are nearly identical and consist of two forms, cationic A1 and anionic A4. In this paper, the organ-specific maturation of the enzyme equipment in these tissues is reported. The activity of arginase in all tissues studied could be detected on the 13th to 16th days of gestation. In fetal intestine and kidney the arginase activity is low, and persists up to the weaning time when the rapid, 10-fold rise of the enzyme activity occurs. However, the adult pattern of arginase isoenzymes in these tissues is accomplished in different ways. In the intestine, arginase A1 appears in fetal life and is the only form of the enzyme till the 19th to 21st days of postnatal life when the second form of arginase, A4, appears and rapidly accumulates, being exclusively responsible for the rise of the total enzyme activity at the time of weaning. In kidney, arginase A1 alone is present in the early fetal period. Arginase A4 appears 3-4 days before birth and its activity persists unchanged within the first 2 weeks of postnatal life. Th intensive rise in total specific activity of kidney arginase at weaning is due to the accumulation of preexisting arginase A4. In brain, the adult pattern of arginase isoenzymes is achieved earlier than in other tissues. Both forms, A1 and A4, occur on Days 13-14 of gestation.