Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse.
Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse.
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发表时间：
1983-09
期刊：
影响因子：
11.2
通讯作者：
A. Kyriazis;W. McCombs;A. Sandberg;A. A. Kyriazis-A.;N. Sloane;R. Lepera
中科院分区：
文献类型：
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作者：
A. Kyriazis;W. McCombs;A. Sandberg;A. A. Kyriazis-A.;N. Sloane;R. Lepera
在组织培养和裸鼠中建立了一种新的肿瘤细胞系，该细胞系来源于一名56岁白人男性的人胰腺导管腺癌。在组织培养中，肿瘤细胞生长为大的、上皮样的、产生粘蛋白的细胞。皮下注射将1 × 10（6）个培养的肿瘤细胞植入裸鼠体内，肿瘤形成在组织学上与原始肿瘤非常相似。超微结构显示肿瘤细胞具有导管上皮特征。在14个遗传决定的位点上建立了该品系的等位酶表型谱，染色体研究显示近四倍体模式。在培养基、肿瘤细胞提取物、裸鼠生长的肿瘤和荷瘤小鼠血清中，乳酸脱氢酶和癌胚抗原等大分子的产生量相对于肿瘤大小可测量。未检测到胰酶。这些特征使得肿瘤细胞系SW-1990成为研究人类胰腺癌生物学各个方面的有价值的工具。
A new tumor line derived from a human pancreatic ductal adenocarcinoma of a 56-year-old Caucasian male was established in tissue culture and the nude mouse. In tissue culture, the neoplastic cells grew as large, epithelial-like, mucin-producing cells. Injection s.c. of 1 X 10(6) cultured neoplastic cells into nude mice resulted in tumor formation histologically closely resembling the original neoplasm. Ultrastructurally, the neoplastic cells showed characteristics of ductal epithelium. The allozyme phenotypic profile of the line was established in 14 genetically determined loci, and chromosome studies showed a near-tetraploid pattern. Production of macromolecules such as lactate dehydrogenase and carcinoembryonic antigen were present in measurable amounts in culture media, tumor cell extracts, nude mouse-grown tumors, and the serum of tumor-bearing mice in amounts relative to tumor size. Pancreatic enzymes were not detected. These characteristics make tumor line SW-1990 a valuable tool in studying various aspects of the biology of human pancreatic cancer.