Alignment of myoblasts on ultrafine gratings inhibits fusion in vitro
Alignment of myoblasts on ultrafine gratings inhibits fusion in vitro
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DOI：
10.1016/s1357-2725(01)00180-7
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发表时间：
2002-07-01
影响因子：
4
通讯作者：
Peckham, M
中科院分区：
文献类型：
--
作者：
Clark, P;Dunn, GA;Peckham, M
在发育过程中，骨骼肌前体细胞融合形成多核肌管。然而，目前还不清楚这种融合是如何调节的，从而产生了线性肌管。在之前的研究中，我们发现在层粘连蛋白微模式上培养的成肌细胞的线形阵列融合成恒定直径的线形肌管。与层粘连蛋白轨道的宽度无关。这表明存在一种机制来防止成肌细胞横向融合[Exp.单元格第230(1997)275号决议]。在这项研究中，我们通过在先前显示的排列成纤维细胞和上皮细胞的超细凹槽表面培养成肌细胞来进一步研究这一点。我们发现，所有单个成肌细胞都沿着沟轴高度排列，时间推移记录显示，运动大多限制在平行于沟槽的方向。然而，与先前的研究相反，早期分化的细胞有强烈的倾向于以大约45度的角度或垂直于沟轴形成聚集体。然而，我们很少看到肌管在这些角度上形成，这支持了我们早先的观点，即细胞横向融合的能力是被禁止的。我们的数据有力地表明，成肌细胞最有可能以端到端的形式融合，正是这种配置使它们能够形成线形而不是不规则的肌管。(C)2002爱思唯尔科学有限公司。保留所有权利。
During development, skeletal muscle precursor cells fuse to form multi-nucleated myotubes. However, it is unclear how this fusion is regulated such that linear myotubes are produced. In a previous study, we found that linear arrays of myoblasts cultured on micropattems of laminin fused to form linear myotubes of a constant diameter. independent of the width of the laminin track. This suggested that a mechanism exists to prevent myoblasts from fusing laterally [Exp. Cell Res. 230 (1997) 275]. In this study, we have investigated this further by culturing myoblasts on ultrafine grooved surfaces previously shown to align fibroblasts and epithelial cells. We found that all the individual myoblasts were highly aligned along the groove axis, and time-lapse recordings showed that motility was mostly restricted to a direction parallel to the grooves. In contrast to the previous study, however, there was a strong tendency for early differentiating cells to form aggregates either at an angle of approximately 45degrees or perpendicular to the groove axis. Nevertheless, we rarely saw myotubes formed at those angles, supporting our earlier idea that the ability of cells to fuse laterally is prohibited. Our data strongly suggest that myoblasts are most likely to fuse in an end-to-end configuration, and it is this that enables them to form linear, rather than irregular myotubes. (C) 2002 Elsevier Science Ltd. All rights reserved.