Cell swelling increased the α2-macroglobulin gene expression in cultured rat hepatocytes
Cell swelling increased the α2-macroglobulin gene expression in cultured rat hepatocytes
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DOI：
10.1016/s0014-5793(98)00038-6
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发表时间：
1998-02-06
期刊：
影响因子：
3.5
通讯作者：
Lavoinne, A
中科院分区：
文献类型：
--
作者：
Meisse, D;Renouf, S;Lavoinne, A
在培养的肝细胞中研究了细胞肿胀对α 2-巨球蛋白（α 2M）基因表达的影响，通过相应刺激α 2M基因的转录速率，低渗诱导α 2M mRNA水平增加（3倍增加），添加棉子糖（100 mM）在mRNA和转录水平上都纠正了低渗的影响。证明细胞肿胀本身是对α 2M基因表达的影响的原因。此外，细胞肿胀的影响是与急性期反应的主要介质白细胞介素6的影响相附加的。(C) 1998年欧洲生化学会联合会。
The effect of cell swelling on the expression of the alpha 2-macroglobulin (alpha 2M) gene was studied in hepatocytes in culture, Hypoosmolarity-induced an increase (3-fold increase) in the level of alpha 2M mRNA through a corresponding stimulation of the rate of transcription of the alpha 2M gene, The addition of raffinose (100 mM) corrected the effect of hypoosmolarity at both mRNA and transcriptional level? demonstrating that cell swelling per se was responsible for the observed effect on the expression of the alpha 2M gene, Moreover, the effect of cell swelling was additive to that of interleukin 6, a major mediator of the acute-phase response. (C) 1998 Federation of European Biochemical Societies.