Transcriptional outputs of the Caenorhabditis elegans forkhead protein DAF-16
Transcriptional outputs of the Caenorhabditis elegans forkhead protein DAF-16
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DOI：
10.1046/j.1474-9728.2003.00043.x
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发表时间：
2003-04-01
期刊：
影响因子：
7.8
通讯作者：
Thomas, JH
中科院分区：
文献类型：
--
作者：
McElwee, J;Bubb, K;Thomas, JH
在秀丽隐杆线虫中，叉头蛋白E1 - 16转导调节幼虫发育和成虫寿命的胰岛素样信号。为了确定在长寿的秀丽隐杆线虫菌株中发生的α-16依赖性转录改变，我们使用cDNA微阵列和基因组分析来确定推定的直接和间接的α-16转录靶基因。我们的分析表明，β-16作用通过改变参与代谢、能量产生和细胞应激反应的基因的表达来调节广泛的生理反应。此外，我们观察到一个很大的重叠之间的α-16依赖的转录和基因通常在长寿的幼虫幼虫阶段表达。最后，我们通过RNA介导的干扰研究了这些靶基因中的35个的体内作用，并确定了一个编码推定的蛋白酶的基因，该蛋白酶是daf-2 Age表型所必需的。
In Caenorhabditis elegans, the forkhead protein DAF-1 6 transduces insulin-like signals that regulate larval development and adult lifespan. To identify DAF-16-dependent transcriptional alterations that occur in a long-lived C elegans strain, we used cDNA microarrays and genomic analysis to identify putative direct and indirect DAF-16 transcriptional target genes. Our analysis suggests that DAF-16 action regulates a wide range of physiological responses by altering the expression of genes involved in metabolism, energy generation and cellular stress responses. Furthermore, we observed a large overlap between DAF-16-dependent transcription and genes normally expressed in the long-lived dauer larval stage. Finally, we examined the in vivo role of 35 of these target genes by RNA-mediated interference and identified one gene encoding a putative protease that is necessary for the daf-2 Age phenotype.