DEVELOPMENT OF ENDOGENOUS PEROXIDASE IN FETAL RAT SUBMANDIBULAR GLAND
DEVELOPMENT OF ENDOGENOUS PEROXIDASE IN FETAL RAT SUBMANDIBULAR GLAND
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胎鼠颌下腺内源性过氧化物酶的发育
DOI：
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发表时间：
1973
影响因子：
3.2
通讯作者：
T. Barka
中科院分区：
文献类型：
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作者：
S. Yamashina;T. Barka
用二氨基联苯胺-H_2O_2组织化学方法研究了大鼠颌下腺内源性过氧化物酶活性的产前发育。16日龄胎儿的下颌下腺由均匀的、未分化的细胞组成，不含分泌颗粒，也没有过氧化物酶活性。过氧化物酶活性在妊娠第17天开始出现于粗面内质网池和少数细胞的核膜。妊娠第18天，在粗面内质网和核膜中显示反应产物的细胞中也含有具有强过氧化物酶活性的分泌颗粒。在妊娠的最后几天，过氧化物酶阳性细胞的数量，其中含有大量的分泌颗粒，增加。含过氧化物酶的细胞是出生后早期前腺泡细胞的直接前体。在同一时期，当含过氧化物酶的细胞分化时，第二种细胞类型也在细胞索中分化。甲苯胺蓝染色的分泌颗粒的出现标志着这种细胞类型的发育。通过产前发育，这种细胞类型显示没有过氧化物酶活性，并与新生儿终末小管细胞。形态学和细胞化学结果表明终末小管细胞和前腺泡细胞为定向细胞，前者向二级闰管细胞分化，后者向成熟腺泡细胞转化。
The prenatal development of endogenous peroxidase activity in the submandibular gland of rat was investigated by means of the diaminobenzidine-H2O2 histochemical method. The submandibular gland of a 16-day-old fetus was composed of cords of uniform, undifferentiated cells which contained no secretory granules and revealed no peroxidase activity. Peroxidase activity first appeared at the 17th day of gestation in the cisternae of the rough endoplasmic reticulum and nuclear envelope in a few cells. At the 18th day of gestation cells which exhibited reaction products in the rough endoplasmic reticulum and nuclear envelope also contained secretory granules with a strong peroxidase activity. During the last days of gestation the number of peroxidase positive cells, which contained numerous secretory granules, increased. The peroxidase-containing cells are the immediate precursors of the proacinar cells of early postnatal stages. During the same time period, when the peroxidase-containing cells differentiated, a second cell type also differentiated in the cellular cords. The development of this cell type was marked by the appearance of secretory granules stainable with toluidine blue. Through the prenatal development, this cell type revealed no peroxidase activity and was identified with the terminal tubule cell of the newborn. The morphologic and cytochemical findings indicate that terminal tubule cells and proacinar cells are committed cells; the former differentiate toward 2nd order intercalated duct cells and the latter transform to mature acinar cells.